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- W2025126327 abstract "Using primer extension, two in vivo transcription start points (tsp) were identified for rightward transcription of the Escherichia coli biotin operon, at nucleotides (nt) +20 and +29. The strongest leftward transcript begins at +9, with a tenfold less abundant transcript starting at +3. The activity of segments cloned into promoter probe vectors locates the major leftward promoter between nt +1 and +105, as expected for the +9 tsp. Although the activity of a chromosomal operator is reduced about 300-fold by point mutation in either arm of the palindrome extending from nt -20 to +20, either a half-operator segment or a full operator bearing the same point mutation in one arm is substantially repressed when cloned into pKB2000, as though cellular location strongly affects the operator's affinity for the repressor." @default.
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- W2025126327 date "1994-07-01" @default.
- W2025126327 modified "2023-09-23" @default.
- W2025126327 title "Transcriptional analysis of the Escherichia coli bio operon" @default.
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- W2025126327 doi "https://doi.org/10.1016/0378-1119(94)90315-8" @default.
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