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- W2025173032 abstract "A double-stranded RNA intermediate has been implicated in the replication of viruses containing a single-stranded RNA genome for animal (Montagnier and Sanders, 1963, Baltimore et al., 1964), bacterial (Weissman et al., 1964, Kelly and Sinsheimer, 1964, Nonoyama and Ikeda, 1964) and plant (Shipp and Haselkorn, 1964) viruses. In most systems, the duplex nature of this species of RNA has been demonstrated indirectly by its resistance to enzymatic cleavage by RNAase, although hyperchromism (Ammann, Delius and Hofschneider, 1964) and X-ray diffraction (Langridge, Billeter, Borst, Burdon and Weissman, 1964) have been utilized when very large quantities were available. Because of the very high melting temperature (90–105°C) exhibited by these structures, some degradation invariably accompanies melting, making it difficult to assess the physical integrity of the virus-like strand and of its compliment. We present here a method for achieving strand separation under mild conditions which does not degrade the single strands thus released, and permits one to examine the duplex structure for single-strand breaks. Using this method, we show that the replicative form of poliovirus RNA, which sediments as a single species at 20S can be completely transformed into a more rapidly sedimenting species, whose behavior is the same as single-stranded RNA extracted from mature virus, sedimenting at 35S." @default.
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- W2025173032 date "1964-12-01" @default.
- W2025173032 modified "2023-09-25" @default.
- W2025173032 title "Two effects of inhibition of protein synthesis on the replication of M12 bacteriophage RNA" @default.
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- W2025173032 doi "https://doi.org/10.1016/s0022-2836(64)80078-4" @default.
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