Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025173608> ?p ?o ?g. }
- W2025173608 endingPage "2770" @default.
- W2025173608 startingPage "2762" @default.
- W2025173608 abstract "Individuals with mutations in the WRN gene suffer from Werner syndrome, a disease with early onset of many characteristics of normal aging. The WRN protein (WRNp) functions in DNA metabolism, as the purified polypeptide has both 3'-->5' helicase and 3'-->5' exonuclease activities. In this study, we have further characterized WRNp exonuclease activity by examining its ability to degrade double-stranded DNA substrates containing abnormal and damaged nucleo-tides. In addition, we directly compared the 3'-->5' WRNp exonuclease activity with that of exo-nuclease III and the Klenow fragment of DNA polymerase I. Our results indicate that the presence of certain abnormal bases (such as uracil and hypoxanthine) does not inhibit the exonuclease activity of WRNp, exo-nuclease III or Klenow, whereas other DNA modifications, including apurinic sites, 8-oxoguanine, 8-oxoadenine and cholesterol adducts, inhibit or block WRNp. The ability of damaged nucleo-tides to inhibit exonucleolytic digestion differs significantly between WRNp, exonuclease III and Klenow, indicating that each exonuclease has a distinct mechanism of action. In addition, normal and modified DNA substrates are degraded similarly by full-length WRNp and an N-terminal fragment of WRNp, indicating that the specificity for this activity lies mostly within this region. The biochemical and physiological significance of these results is discussed." @default.
- W2025173608 created "2016-06-24" @default.
- W2025173608 creator A5023246308 @default.
- W2025173608 creator A5061742718 @default.
- W2025173608 creator A5089475983 @default.
- W2025173608 creator A5089917586 @default.
- W2025173608 date "2000-07-15" @default.
- W2025173608 modified "2023-10-01" @default.
- W2025173608 title "Selective blockage of the 3'->5' exonuclease activity of WRN protein by certain oxidative modifications and bulky lesions in DNA" @default.
- W2025173608 cites W1481052392 @default.
- W2025173608 cites W1538603370 @default.
- W2025173608 cites W1542472052 @default.
- W2025173608 cites W1545526472 @default.
- W2025173608 cites W1565120973 @default.
- W2025173608 cites W159681246 @default.
- W2025173608 cites W1935801587 @default.
- W2025173608 cites W1940074648 @default.
- W2025173608 cites W1966347623 @default.
- W2025173608 cites W1967336537 @default.
- W2025173608 cites W1969578702 @default.
- W2025173608 cites W1970285696 @default.
- W2025173608 cites W1970898019 @default.
- W2025173608 cites W1973751760 @default.
- W2025173608 cites W1975817860 @default.
- W2025173608 cites W1975853177 @default.
- W2025173608 cites W1979664563 @default.
- W2025173608 cites W1987623573 @default.
- W2025173608 cites W1994166791 @default.
- W2025173608 cites W1995702418 @default.
- W2025173608 cites W2008669260 @default.
- W2025173608 cites W2023612394 @default.
- W2025173608 cites W2031184810 @default.
- W2025173608 cites W2035650458 @default.
- W2025173608 cites W2039996950 @default.
- W2025173608 cites W2049160479 @default.
- W2025173608 cites W2050717008 @default.
- W2025173608 cites W2059501883 @default.
- W2025173608 cites W2064310741 @default.
- W2025173608 cites W2071924188 @default.
- W2025173608 cites W2082767985 @default.
- W2025173608 cites W2084712319 @default.
- W2025173608 cites W2106295681 @default.
- W2025173608 cites W2127460704 @default.
- W2025173608 cites W2138524348 @default.
- W2025173608 cites W2153043528 @default.
- W2025173608 cites W2160929874 @default.
- W2025173608 cites W4248084843 @default.
- W2025173608 doi "https://doi.org/10.1093/nar/28.14.2762" @default.
- W2025173608 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/102646" @default.
- W2025173608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10908333" @default.
- W2025173608 hasPublicationYear "2000" @default.
- W2025173608 type Work @default.
- W2025173608 sameAs 2025173608 @default.
- W2025173608 citedByCount "60" @default.
- W2025173608 countsByYear W20251736082012 @default.
- W2025173608 countsByYear W20251736082013 @default.
- W2025173608 countsByYear W20251736082014 @default.
- W2025173608 countsByYear W20251736082015 @default.
- W2025173608 countsByYear W20251736082016 @default.
- W2025173608 countsByYear W20251736082018 @default.
- W2025173608 countsByYear W20251736082019 @default.
- W2025173608 countsByYear W20251736082022 @default.
- W2025173608 countsByYear W20251736082023 @default.
- W2025173608 crossrefType "journal-article" @default.
- W2025173608 hasAuthorship W2025173608A5023246308 @default.
- W2025173608 hasAuthorship W2025173608A5061742718 @default.
- W2025173608 hasAuthorship W2025173608A5089475983 @default.
- W2025173608 hasAuthorship W2025173608A5089917586 @default.
- W2025173608 hasBestOaLocation W20251736081 @default.
- W2025173608 hasConcept C104317684 @default.
- W2025173608 hasConcept C134935766 @default.
- W2025173608 hasConcept C150777479 @default.
- W2025173608 hasConcept C153911025 @default.
- W2025173608 hasConcept C161223559 @default.
- W2025173608 hasConcept C2756471 @default.
- W2025173608 hasConcept C2777271071 @default.
- W2025173608 hasConcept C2777501222 @default.
- W2025173608 hasConcept C54415193 @default.
- W2025173608 hasConcept C552990157 @default.
- W2025173608 hasConcept C55493867 @default.
- W2025173608 hasConcept C67705224 @default.
- W2025173608 hasConcept C86803240 @default.
- W2025173608 hasConceptScore W2025173608C104317684 @default.
- W2025173608 hasConceptScore W2025173608C134935766 @default.
- W2025173608 hasConceptScore W2025173608C150777479 @default.
- W2025173608 hasConceptScore W2025173608C153911025 @default.
- W2025173608 hasConceptScore W2025173608C161223559 @default.
- W2025173608 hasConceptScore W2025173608C2756471 @default.
- W2025173608 hasConceptScore W2025173608C2777271071 @default.
- W2025173608 hasConceptScore W2025173608C2777501222 @default.
- W2025173608 hasConceptScore W2025173608C54415193 @default.
- W2025173608 hasConceptScore W2025173608C552990157 @default.
- W2025173608 hasConceptScore W2025173608C55493867 @default.
- W2025173608 hasConceptScore W2025173608C67705224 @default.
- W2025173608 hasConceptScore W2025173608C86803240 @default.
- W2025173608 hasIssue "14" @default.
- W2025173608 hasLocation W20251736081 @default.
- W2025173608 hasLocation W20251736082 @default.