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• W2025232009 startingPage "2110" @default.
• W2025232009 abstract "The 3′ strand of telomeres is composed of tandem repeats of short G-rich sequences which protrude as single-stranded DNA overhangs. These repeats are G3T2A in humans and G4T2 and G4T4 in the ciliates Tetrahymena and Oxytricha, respectively. We analyzed different quadruplex-forming sequences derived from telomeric sequences, G3+k(Tn+kG3+k)3 and G3+k(T2AG3+k)3, in the presence of Li+, Na+, and K+ through the use of circular dichroism, UV spectroscopy, and electrophoresis, where k = 0 or 1 and n = 1−3. Results obtained under the given conditions can provide more detailed information about the quadruplex structure. The major findings are as follows. (i) G-Repeats in solution form a mix of topologically different structures; only G3(T2G3)3 and G3(TG3)3 repeats preferentially form the parallel interstrand structure. (ii) The Tetrahymena repeat can form at least two intramolecular conformers with different strand orientations and levels of stability. (iii) G-Quadruplex conformation and molecularity strongly depend on the type and concentration of ions used in the solution. The formation of intramolecular quadruplexes is governed by the length of the loops connecting G-runs. Intermolecular G-quadruplex forms are more likely to form in a higher concentration of ions for sequences where G-runs are separated by only one or two nucleotides." @default.
• W2025232009 created "2016-06-24" @default.
• W2025232009 creator A5020928328 @default.
• W2025232009 creator A5030333935 @default.
• W2025232009 creator A5052487553 @default.
• W2025232009 date "2010-02-17" @default.
• W2025232009 modified "2023-09-26" @default.
• W2025232009 title "Structural Features of Intra- and Intermolecular G-Quadruplexes Derived from Telomeric Repeats" @default.
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• W2025232009 doi "https://doi.org/10.1021/bi902099u" @default.
• W2025232009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20143878" @default.
• W2025232009 hasPublicationYear "2010" @default.
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