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• W2025255044 endingPage "311" @default.
• W2025255044 startingPage "298" @default.
• W2025255044 abstract "Reduction of a cytochrome b following excitation by a single, short, near-saturating light flash has been demonstrated in Chromatium vinosum chromatophores. The extent of reduction is increased by addition of antimycin. The cytochrome has an alpha-band maximum at 562 nm in the presence of antimycin. The cytochrome b reduction is most readily observed in the presence of antimycin at high redox potential when cytochrome c-555 is oxidised before excitation. Under these conditions the half-time for reduction is about 20 ms, and the extent is about 0.5 mol of cytochrome b reduced per mol of reaction center oxidised. This extent of reduction is observed on the first flash-excitation from the dark-adapted state, and there was no indication that the reaction center quinone acceptor complex acted as a two-electron accumulating system. With cytochrome c-555 reduced before excitation, the extent of cytochrome b reduction is approximately halved. The factors which result in substoichiometric cytochrome b reduction are not yet understood. Agents which appear to inhibit primary acceptor oxidation by the secondary acceptor (UHDBT, PHDBT, DDAQQ, HOQNO, o-phenanthroline), inhibit reduction of the cytochrome b. DBMIB inhibits cytochrome b reduction but does not appear to inhibit primary acceptor oxidation. These observations confirm that a cytochrome b receives electrons delivered from the primary acceptor complex, and indicate that the photoreduced cytochrome b is reoxidised via an antimycin-sensitive pathway." @default.
• W2025255044 created "2016-06-24" @default.
• W2025255044 creator A5048072241 @default.
• W2025255044 creator A5078991075 @default.
• W2025255044 date "1980-07-01" @default.
• W2025255044 modified "2023-10-16" @default.
• W2025255044 title "The photosynthetic electron transfer chain of Chromatium vinosum chromatophores flash-induced cytochrome b reduction" @default.
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• W2025255044 doi "https://doi.org/10.1016/0005-2728(80)90161-9" @default.
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• W2025255044 hasPublicationYear "1980" @default.
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