Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025256893> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2025256893 endingPage "711" @default.
- W2025256893 startingPage "704" @default.
- W2025256893 abstract "Bleomycin (BLM), a well-known DNA scission agent, is assumed to inhibit intracellular DNA replication by damaging the DNA template (cis-acting mechanism), although other DNA damaging compounds can alter DNA replication through modulation of crucial replication factor(s) (trans-acting mechanism). The present study examines the relationship between DNA damage and inhibition of replication caused by BLM in the well-defined simian virus 40 (SV40) intracellular and cell-free in vitro systems. Treatment of SV40-infected BSC-1 cells for 2 h with BLM at 50 microg/mL, induced 0.3 break/viral genome. Under the same treatment conditions, analysis of replication intermediates on two-dimensional gels showed a decrease in both mass of SV40 replication intermediates and replication activity. The mass of SV40 intermediates was decreased to about 30%, whereas replication activity was reduced to less than 5%. These results suggest that BLM inhibits both initiation and elongation phases of SV40 replication. In a cell-free DNA replication system, extracts from BLM-treated cells (50 micro/mL) were able to support SV40 DNA replication by only 50%. In this study, non-drug-treated DNA template was used, implying that BLM can induce a trans-acting effect. Finally, the drug-induced effects on SV40 DNA replication in cell-free and intracellular viral systems were compared to the effects on genomic DNA replication in BSC-1 cells. Overall, the results support the concept that BLM-induced inhibition of DNA replication occurs by both trans- (inhibition of replication of nondamaged template) and cis-acting mechanisms (template damage)." @default.
- W2025256893 created "2016-06-24" @default.
- W2025256893 creator A5029365707 @default.
- W2025256893 creator A5070880248 @default.
- W2025256893 creator A5080046324 @default.
- W2025256893 date "2000-12-21" @default.
- W2025256893 modified "2023-10-15" @default.
- W2025256893 title "Bleomycin-Induced Alterations in DNA Replication: Relationship to DNA Damage" @default.
- W2025256893 cites W1493806519 @default.
- W2025256893 cites W1972126192 @default.
- W2025256893 cites W2042732063 @default.
- W2025256893 cites W2105908798 @default.
- W2025256893 cites W2137026987 @default.
- W2025256893 cites W2147689241 @default.
- W2025256893 cites W2415217075 @default.
- W2025256893 cites W2470173456 @default.
- W2025256893 cites W28829384 @default.
- W2025256893 doi "https://doi.org/10.1021/bi002181n" @default.
- W2025256893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11170387" @default.
- W2025256893 hasPublicationYear "2000" @default.
- W2025256893 type Work @default.
- W2025256893 sameAs 2025256893 @default.
- W2025256893 citedByCount "9" @default.
- W2025256893 countsByYear W20252568932012 @default.
- W2025256893 countsByYear W20252568932016 @default.
- W2025256893 countsByYear W20252568932017 @default.
- W2025256893 countsByYear W20252568932023 @default.
- W2025256893 crossrefType "journal-article" @default.
- W2025256893 hasAuthorship W2025256893A5029365707 @default.
- W2025256893 hasAuthorship W2025256893A5070880248 @default.
- W2025256893 hasAuthorship W2025256893A5080046324 @default.
- W2025256893 hasConcept C104317684 @default.
- W2025256893 hasConcept C104690221 @default.
- W2025256893 hasConcept C113271649 @default.
- W2025256893 hasConcept C140704245 @default.
- W2025256893 hasConcept C151158763 @default.
- W2025256893 hasConcept C153911025 @default.
- W2025256893 hasConcept C159047783 @default.
- W2025256893 hasConcept C185592680 @default.
- W2025256893 hasConcept C188036394 @default.
- W2025256893 hasConcept C2522874641 @default.
- W2025256893 hasConcept C552990157 @default.
- W2025256893 hasConcept C55493867 @default.
- W2025256893 hasConcept C73573662 @default.
- W2025256893 hasConcept C86339819 @default.
- W2025256893 hasConcept C86803240 @default.
- W2025256893 hasConcept C94966510 @default.
- W2025256893 hasConcept C95444343 @default.
- W2025256893 hasConcept C98086919 @default.
- W2025256893 hasConceptScore W2025256893C104317684 @default.
- W2025256893 hasConceptScore W2025256893C104690221 @default.
- W2025256893 hasConceptScore W2025256893C113271649 @default.
- W2025256893 hasConceptScore W2025256893C140704245 @default.
- W2025256893 hasConceptScore W2025256893C151158763 @default.
- W2025256893 hasConceptScore W2025256893C153911025 @default.
- W2025256893 hasConceptScore W2025256893C159047783 @default.
- W2025256893 hasConceptScore W2025256893C185592680 @default.
- W2025256893 hasConceptScore W2025256893C188036394 @default.
- W2025256893 hasConceptScore W2025256893C2522874641 @default.
- W2025256893 hasConceptScore W2025256893C552990157 @default.
- W2025256893 hasConceptScore W2025256893C55493867 @default.
- W2025256893 hasConceptScore W2025256893C73573662 @default.
- W2025256893 hasConceptScore W2025256893C86339819 @default.
- W2025256893 hasConceptScore W2025256893C86803240 @default.
- W2025256893 hasConceptScore W2025256893C94966510 @default.
- W2025256893 hasConceptScore W2025256893C95444343 @default.
- W2025256893 hasConceptScore W2025256893C98086919 @default.
- W2025256893 hasIssue "3" @default.
- W2025256893 hasLocation W20252568931 @default.
- W2025256893 hasLocation W20252568932 @default.
- W2025256893 hasOpenAccess W2025256893 @default.
- W2025256893 hasPrimaryLocation W20252568931 @default.
- W2025256893 hasRelatedWork W1916339093 @default.
- W2025256893 hasRelatedWork W2002372083 @default.
- W2025256893 hasRelatedWork W2008783898 @default.
- W2025256893 hasRelatedWork W2035892621 @default.
- W2025256893 hasRelatedWork W2067650917 @default.
- W2025256893 hasRelatedWork W2068761725 @default.
- W2025256893 hasRelatedWork W2157888312 @default.
- W2025256893 hasRelatedWork W4247201266 @default.
- W2025256893 hasRelatedWork W4298619437 @default.
- W2025256893 hasRelatedWork W4387233873 @default.
- W2025256893 hasVolume "40" @default.
- W2025256893 isParatext "false" @default.
- W2025256893 isRetracted "false" @default.
- W2025256893 magId "2025256893" @default.
- W2025256893 workType "article" @default.