Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025259610> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2025259610 endingPage "64" @default.
- W2025259610 startingPage "57" @default.
- W2025259610 abstract "Most studies of erythropoiesis in vitro have employed cloning methods in semisolid medium. We have recently described a two-step liquid culture procedure that supports the proliferation and differentiation of human erythroid progenitors. In the present study, we have modified the procedure to allow large-scale cultures of erythroid cells derived from normal donors. The culture is divided into two phases. In the first phase, which is erythropoietin (Epo) independent, the early erythroid progenitors multiply and differentiate. In the second, Epo-dependent phase, they mature into orthochromatic normoblasts and enucleated erythrocytes. Using this procedure, erythroid cell yield reached 7.5 × 106/ml and a total of 7 × 108 cells could be harvested per blood unit. A comparison of the growth of erythroid cells in liquid culture to their colony growth in semisolid culture indicated that cell growth was superior: 1) in liquid culture in terms of cell yield per originally cultured mononuclear cell, 2) per ml culture and per culture surface area and in the purity of the resultant erythroid cell population. In addition, it permits easier manipulation of the culture condition and components and sampling of > 1 × 107 cells at each maturation stage subsequent to the proerythroblast stage. This liquid culture procedure might provide an important experimental tool for studying erythroid cell development." @default.
- W2025259610 created "2016-06-24" @default.
- W2025259610 creator A5015469728 @default.
- W2025259610 creator A5034865472 @default.
- W2025259610 creator A5041022680 @default.
- W2025259610 creator A5083807634 @default.
- W2025259610 date "1991-01-01" @default.
- W2025259610 modified "2023-09-29" @default.
- W2025259610 title "Growth of human normal erythroid progenitors in liquid culture: A comparison with colony growth in semisolid culture" @default.
- W2025259610 cites W137255699 @default.
- W2025259610 cites W1516165231 @default.
- W2025259610 cites W1979704489 @default.
- W2025259610 cites W1991206650 @default.
- W2025259610 cites W1994005628 @default.
- W2025259610 cites W2039820478 @default.
- W2025259610 cites W307574227 @default.
- W2025259610 cites W4236429009 @default.
- W2025259610 cites W4239139991 @default.
- W2025259610 cites W4249832396 @default.
- W2025259610 cites W64258421 @default.
- W2025259610 cites W85233391 @default.
- W2025259610 doi "https://doi.org/10.1002/stem.5530090108" @default.
- W2025259610 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2010656" @default.
- W2025259610 hasPublicationYear "1991" @default.
- W2025259610 type Work @default.
- W2025259610 sameAs 2025259610 @default.
- W2025259610 citedByCount "51" @default.
- W2025259610 countsByYear W20252596102013 @default.
- W2025259610 countsByYear W20252596102014 @default.
- W2025259610 countsByYear W20252596102016 @default.
- W2025259610 crossrefType "journal-article" @default.
- W2025259610 hasAuthorship W2025259610A5015469728 @default.
- W2025259610 hasAuthorship W2025259610A5034865472 @default.
- W2025259610 hasAuthorship W2025259610A5041022680 @default.
- W2025259610 hasAuthorship W2025259610A5083807634 @default.
- W2025259610 hasConcept C126322002 @default.
- W2025259610 hasConcept C134018914 @default.
- W2025259610 hasConcept C16685009 @default.
- W2025259610 hasConcept C201750760 @default.
- W2025259610 hasConcept C202751555 @default.
- W2025259610 hasConcept C203014093 @default.
- W2025259610 hasConcept C2778248108 @default.
- W2025259610 hasConcept C2778534260 @default.
- W2025259610 hasConcept C2779703530 @default.
- W2025259610 hasConcept C28328180 @default.
- W2025259610 hasConcept C2908647359 @default.
- W2025259610 hasConcept C54355233 @default.
- W2025259610 hasConcept C55493867 @default.
- W2025259610 hasConcept C62112901 @default.
- W2025259610 hasConcept C71924100 @default.
- W2025259610 hasConcept C81885089 @default.
- W2025259610 hasConcept C86803240 @default.
- W2025259610 hasConcept C95444343 @default.
- W2025259610 hasConcept C99454951 @default.
- W2025259610 hasConceptScore W2025259610C126322002 @default.
- W2025259610 hasConceptScore W2025259610C134018914 @default.
- W2025259610 hasConceptScore W2025259610C16685009 @default.
- W2025259610 hasConceptScore W2025259610C201750760 @default.
- W2025259610 hasConceptScore W2025259610C202751555 @default.
- W2025259610 hasConceptScore W2025259610C203014093 @default.
- W2025259610 hasConceptScore W2025259610C2778248108 @default.
- W2025259610 hasConceptScore W2025259610C2778534260 @default.
- W2025259610 hasConceptScore W2025259610C2779703530 @default.
- W2025259610 hasConceptScore W2025259610C28328180 @default.
- W2025259610 hasConceptScore W2025259610C2908647359 @default.
- W2025259610 hasConceptScore W2025259610C54355233 @default.
- W2025259610 hasConceptScore W2025259610C55493867 @default.
- W2025259610 hasConceptScore W2025259610C62112901 @default.
- W2025259610 hasConceptScore W2025259610C71924100 @default.
- W2025259610 hasConceptScore W2025259610C81885089 @default.
- W2025259610 hasConceptScore W2025259610C86803240 @default.
- W2025259610 hasConceptScore W2025259610C95444343 @default.
- W2025259610 hasConceptScore W2025259610C99454951 @default.
- W2025259610 hasIssue "1" @default.
- W2025259610 hasLocation W20252596101 @default.
- W2025259610 hasLocation W20252596102 @default.
- W2025259610 hasOpenAccess W2025259610 @default.
- W2025259610 hasPrimaryLocation W20252596101 @default.
- W2025259610 hasRelatedWork W1967805100 @default.
- W2025259610 hasRelatedWork W2009506003 @default.
- W2025259610 hasRelatedWork W2026452824 @default.
- W2025259610 hasRelatedWork W2046644892 @default.
- W2025259610 hasRelatedWork W2122248575 @default.
- W2025259610 hasRelatedWork W2283185949 @default.
- W2025259610 hasRelatedWork W2411457118 @default.
- W2025259610 hasRelatedWork W2414506550 @default.
- W2025259610 hasRelatedWork W2465035677 @default.
- W2025259610 hasRelatedWork W2594242553 @default.
- W2025259610 hasVolume "9" @default.
- W2025259610 isParatext "false" @default.
- W2025259610 isRetracted "false" @default.
- W2025259610 magId "2025259610" @default.
- W2025259610 workType "article" @default.