Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025277347> ?p ?o ?g. }
- W2025277347 endingPage "e191" @default.
- W2025277347 startingPage "e191" @default.
- W2025277347 abstract "Although much effort has been directed at dissecting the mechanisms of central tolerance, the role of thymic stromal cells remains elusive. In order to further characterize this event, we developed a mouse model restricting LacZ to thymic stromal cotransporter (TSCOT)-expressing thymic stromal cells (TDLacZ). The thymus of this mouse contains approximately 4,300 TSCOT+ cells, each expressing several thousand molecules of the LacZ antigen. TSCOT+ cells express the cortical marker CDR1, CD40, CD80, CD54, and major histocompatibility complex class II (MHCII). When examining endogenous responses directed against LacZ, we observed significant tolerance. This was evidenced in a diverse T cell repertoire as measured by both a CD4 T cell proliferation assay and an antigen-specific antibody isotype analysis. This tolerance process was at least partially independent of Autoimmune Regulatory Element gene expression. When TDLacZ mice were crossed to a novel CD4 T cell receptor (TCR) transgenic reactive against LacZ (BgII), there was a complete deletion of double-positive thymocytes. Fetal thymic reaggregate culture of CD45- and UEA-depleted thymic stromal cells from TDLacZ and sorted TCR-bearing thymocytes excluded the possibility of cross presentation by thymic dendritic cells and medullary epithelial cells for the deletion. Overall, these results demonstrate that the introduction of a neoantigen into TSCOT-expressing cells can efficiently establish complete tolerance and suggest a possible application for the deletion of antigen-specific T cells by antigen introduction into TSCOT+ cells." @default.
- W2025277347 created "2016-06-24" @default.
- W2025277347 creator A5000045224 @default.
- W2025277347 creator A5010043674 @default.
- W2025277347 creator A5020632910 @default.
- W2025277347 creator A5028095065 @default.
- W2025277347 creator A5056074234 @default.
- W2025277347 creator A5061987607 @default.
- W2025277347 creator A5069005159 @default.
- W2025277347 creator A5069297078 @default.
- W2025277347 creator A5074382439 @default.
- W2025277347 creator A5077073905 @default.
- W2025277347 creator A5077409768 @default.
- W2025277347 creator A5082487657 @default.
- W2025277347 creator A5083338142 @default.
- W2025277347 creator A5090305621 @default.
- W2025277347 date "2008-08-05" @default.
- W2025277347 modified "2023-09-27" @default.
- W2025277347 title "TSCOT + Thymic Epithelial Cell-Mediated Sensitive CD4 Tolerance by Direct Presentation" @default.
- W2025277347 cites W114475871 @default.
- W2025277347 cites W1516518394 @default.
- W2025277347 cites W1555988808 @default.
- W2025277347 cites W155806725 @default.
- W2025277347 cites W1576917530 @default.
- W2025277347 cites W1578427177 @default.
- W2025277347 cites W1615518152 @default.
- W2025277347 cites W1957599787 @default.
- W2025277347 cites W1965882358 @default.
- W2025277347 cites W1969405649 @default.
- W2025277347 cites W1969925560 @default.
- W2025277347 cites W1971816369 @default.
- W2025277347 cites W1973822200 @default.
- W2025277347 cites W1979133688 @default.
- W2025277347 cites W1980521737 @default.
- W2025277347 cites W1982599502 @default.
- W2025277347 cites W1983113497 @default.
- W2025277347 cites W1983265298 @default.
- W2025277347 cites W1983336399 @default.
- W2025277347 cites W1987987231 @default.
- W2025277347 cites W1991925617 @default.
- W2025277347 cites W1993153481 @default.
- W2025277347 cites W1997887701 @default.
- W2025277347 cites W1998197496 @default.
- W2025277347 cites W1998821335 @default.
- W2025277347 cites W1999341695 @default.
- W2025277347 cites W1999928832 @default.
- W2025277347 cites W2000199728 @default.
- W2025277347 cites W2003063140 @default.
- W2025277347 cites W2004282938 @default.
- W2025277347 cites W2008582777 @default.
- W2025277347 cites W2012125630 @default.
- W2025277347 cites W2012324563 @default.
- W2025277347 cites W2014228101 @default.
- W2025277347 cites W2014924137 @default.
- W2025277347 cites W2018214174 @default.
- W2025277347 cites W2019522474 @default.
- W2025277347 cites W2027844464 @default.
- W2025277347 cites W2030216946 @default.
- W2025277347 cites W2035910941 @default.
- W2025277347 cites W2036613884 @default.
- W2025277347 cites W2043947355 @default.
- W2025277347 cites W2044285203 @default.
- W2025277347 cites W2045008512 @default.
- W2025277347 cites W2058401819 @default.
- W2025277347 cites W2060827478 @default.
- W2025277347 cites W2069664665 @default.
- W2025277347 cites W2070706765 @default.
- W2025277347 cites W2073474757 @default.
- W2025277347 cites W2083095889 @default.
- W2025277347 cites W2089154010 @default.
- W2025277347 cites W2100890363 @default.
- W2025277347 cites W2106945792 @default.
- W2025277347 cites W2112506009 @default.
- W2025277347 cites W2114569569 @default.
- W2025277347 cites W2115771911 @default.
- W2025277347 cites W2122852190 @default.
- W2025277347 cites W2125540086 @default.
- W2025277347 cites W2125592352 @default.
- W2025277347 cites W2125981766 @default.
- W2025277347 cites W2130478147 @default.
- W2025277347 cites W2138319166 @default.
- W2025277347 cites W2140489687 @default.
- W2025277347 cites W2145736817 @default.
- W2025277347 cites W2155778479 @default.
- W2025277347 cites W2159573388 @default.
- W2025277347 cites W2160127445 @default.
- W2025277347 cites W2161371315 @default.
- W2025277347 cites W2165214298 @default.
- W2025277347 cites W2174800991 @default.
- W2025277347 cites W2022893679 @default.
- W2025277347 doi "https://doi.org/10.1371/journal.pbio.0060191" @default.
- W2025277347 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2494558" @default.
- W2025277347 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18684012" @default.
- W2025277347 hasPublicationYear "2008" @default.
- W2025277347 type Work @default.
- W2025277347 sameAs 2025277347 @default.
- W2025277347 citedByCount "17" @default.
- W2025277347 countsByYear W20252773472012 @default.