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- W2025286664 abstract "In recent years there has been great interest in quantitative polymerase chain reaction. Consequently, a large number of assays have been developed, of which the one using non-homologous competitors is arguably the most precise. Despite widespread applications, currently there is no simple method to synthesize such competitors. Here a facile and cost-effective, single-step method for synthesis of recombinant, non-homologous, competitor complementary DNA is described. The method can be adapted to generate competitors of any size and sequence. The entire procedure is quick, straightforward and does not require any specialized equipment except a standard thermocycler." @default.
- W2025286664 created "2016-06-24" @default.
- W2025286664 creator A5012662598 @default.
- W2025286664 date "2002-01-17" @default.
- W2025286664 modified "2023-10-14" @default.
- W2025286664 title "A Simple Single-Step Method for the Synthesis of Recombinant Non-Homologous Competitor cDNA and Its Implications for Quantitative PCR in Biological Sciences and Molecular Diagnostics" @default.
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- W2025286664 doi "https://doi.org/10.1515/bc.2002.167" @default.
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