Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025295622> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2025295622 endingPage "120" @default.
- W2025295622 startingPage "114" @default.
- W2025295622 abstract "Membrane-bound [NiFe]-hydrogenase from Hydrogenophaga sp. AH-24 was purified to homogeneity. The molecular weight was estimated as 100±10 kDa, consisting of two different subunits (62 and 37 kDa). The optimal pH values for H2 oxidation and evolution were 8.0 and 4.0, respectively, and the activity ratio (H2 oxidation/H2 evolution) was 1.61 × 102 at pH 7.0. The optimal temperature was 75 °C. The enzyme was quite stable under air atmosphere (the half-life of activity was c. 48 h at 4 °C), which should be important to function in the aerobic habitat of the strain. The enzyme showed high thermal stability under anaerobic conditions, which retained full activity for over 5 h at 50 °C. The activity increased up to 2.5-fold during incubation at 50 °C under H2. Using methylene blue as an electron acceptor, the kinetic constants of the purified membrane-bound homogenase (MBH) were Vmax=336 U mg−1, kcat=560 s−1, and kcat/Km=2.24 × 107 M−1 s−1. The MBH exhibited prominent electron paramagnetic resonance signals originating from [3Fe–4S]+ and [4Fe–4S]+ clusters. On the other hand, signals originating from Ni of the active center were very weak, as observed in other oxygen-stable hydrogenases from aerobic H2-oxidizing bacteria. This is the first report of catalytic and biochemical characterization of the respiratory MBH from Hydrogenophaga." @default.
- W2025295622 created "2016-06-24" @default.
- W2025295622 creator A5027995745 @default.
- W2025295622 creator A5032182445 @default.
- W2025295622 creator A5044561362 @default.
- W2025295622 creator A5067741202 @default.
- W2025295622 creator A5091565150 @default.
- W2025295622 date "2009-01-01" @default.
- W2025295622 modified "2023-09-26" @default.
- W2025295622 title "Purification and biochemical characterization of a membrane-bound [NiFe]-hydrogenase from a hydrogen-oxidizing, lithotrophic bacterium,<i>Hydrogenophaga</i>sp. AH-24" @default.
- W2025295622 cites W1755717076 @default.
- W2025295622 cites W1974649620 @default.
- W2025295622 cites W1975909805 @default.
- W2025295622 cites W1983925681 @default.
- W2025295622 cites W1992007952 @default.
- W2025295622 cites W2080669830 @default.
- W2025295622 cites W2116403749 @default.
- W2025295622 cites W2128988319 @default.
- W2025295622 cites W2170154121 @default.
- W2025295622 cites W4293247451 @default.
- W2025295622 doi "https://doi.org/10.1111/j.1574-6968.2008.01417.x" @default.
- W2025295622 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19025569" @default.
- W2025295622 hasPublicationYear "2009" @default.
- W2025295622 type Work @default.
- W2025295622 sameAs 2025295622 @default.
- W2025295622 citedByCount "14" @default.
- W2025295622 countsByYear W20252956222012 @default.
- W2025295622 countsByYear W20252956222014 @default.
- W2025295622 countsByYear W20252956222015 @default.
- W2025295622 countsByYear W20252956222016 @default.
- W2025295622 countsByYear W20252956222017 @default.
- W2025295622 countsByYear W20252956222021 @default.
- W2025295622 countsByYear W20252956222022 @default.
- W2025295622 countsByYear W20252956222023 @default.
- W2025295622 crossrefType "journal-article" @default.
- W2025295622 hasAuthorship W2025295622A5027995745 @default.
- W2025295622 hasAuthorship W2025295622A5032182445 @default.
- W2025295622 hasAuthorship W2025295622A5044561362 @default.
- W2025295622 hasAuthorship W2025295622A5067741202 @default.
- W2025295622 hasAuthorship W2025295622A5091565150 @default.
- W2025295622 hasConcept C13965031 @default.
- W2025295622 hasConcept C178790620 @default.
- W2025295622 hasConcept C179104552 @default.
- W2025295622 hasConcept C181199279 @default.
- W2025295622 hasConcept C185592680 @default.
- W2025295622 hasConcept C2900893 @default.
- W2025295622 hasConcept C41183919 @default.
- W2025295622 hasConcept C523546767 @default.
- W2025295622 hasConcept C54355233 @default.
- W2025295622 hasConcept C55493867 @default.
- W2025295622 hasConcept C56856141 @default.
- W2025295622 hasConcept C77176794 @default.
- W2025295622 hasConcept C79115784 @default.
- W2025295622 hasConcept C86803240 @default.
- W2025295622 hasConceptScore W2025295622C13965031 @default.
- W2025295622 hasConceptScore W2025295622C178790620 @default.
- W2025295622 hasConceptScore W2025295622C179104552 @default.
- W2025295622 hasConceptScore W2025295622C181199279 @default.
- W2025295622 hasConceptScore W2025295622C185592680 @default.
- W2025295622 hasConceptScore W2025295622C2900893 @default.
- W2025295622 hasConceptScore W2025295622C41183919 @default.
- W2025295622 hasConceptScore W2025295622C523546767 @default.
- W2025295622 hasConceptScore W2025295622C54355233 @default.
- W2025295622 hasConceptScore W2025295622C55493867 @default.
- W2025295622 hasConceptScore W2025295622C56856141 @default.
- W2025295622 hasConceptScore W2025295622C77176794 @default.
- W2025295622 hasConceptScore W2025295622C79115784 @default.
- W2025295622 hasConceptScore W2025295622C86803240 @default.
- W2025295622 hasIssue "1" @default.
- W2025295622 hasLocation W20252956221 @default.
- W2025295622 hasLocation W20252956222 @default.
- W2025295622 hasOpenAccess W2025295622 @default.
- W2025295622 hasPrimaryLocation W20252956221 @default.
- W2025295622 hasRelatedWork W144097882 @default.
- W2025295622 hasRelatedWork W1573048935 @default.
- W2025295622 hasRelatedWork W1973011078 @default.
- W2025295622 hasRelatedWork W2029001174 @default.
- W2025295622 hasRelatedWork W2047434710 @default.
- W2025295622 hasRelatedWork W2103949734 @default.
- W2025295622 hasRelatedWork W2400672345 @default.
- W2025295622 hasRelatedWork W2753742277 @default.
- W2025295622 hasRelatedWork W2949422613 @default.
- W2025295622 hasRelatedWork W4255220560 @default.
- W2025295622 hasVolume "290" @default.
- W2025295622 isParatext "false" @default.
- W2025295622 isRetracted "false" @default.
- W2025295622 magId "2025295622" @default.
- W2025295622 workType "article" @default.