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- W2025300827 abstract "The Barnase-like Domain in DNA-Directed RNA Polymerase II of Saccharomyces cerevisiae containing three cysteine residues was synthesized by a thioester method. A partially-protected cysteine-containing peptide thioester was prepared via a peptide obtained by a solid-phase method using 3-nitro-2-pyridinesulfenyl amino acid derivatives. Four peptide segments covering 112 amino acid residues of the barnase-like domain were condensed from the C-terminal to the N-terminal one by one in the presence of silver ions and N-hydroxysuccinimide to give a full sequence peptide. RNase activity of the synthetic domain could not be detected because of its extreme low solubility in the neutral buffer." @default.
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- W2025300827 date "1995-01-01" @default.
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- W2025300827 title "Preparation of<i>S</i>-Protected Cysteine-Containing Peptide Thioester and Its Use for the Synthesis of the Barnase-Like Domain in DNA-Directed RNA Polymerase II of<i>Saccharomyces cerevisiae</i>" @default.
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- W2025300827 doi "https://doi.org/10.1246/bcsj.68.330" @default.
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