Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025366145> ?p ?o ?g. }
- W2025366145 endingPage "392" @default.
- W2025366145 startingPage "383" @default.
- W2025366145 abstract "Alternative assays are highly desirable to reduce the extensive experimental animal use in developmental toxicity testing. In the present study, we developed an improved test system for assessing neurodevelopmental toxicity using differentiating embryonic stem cells. We advanced previously established methods by merging, modifying and abbreviating the original 20-day protocol into a more efficient 13-day neural differentiation protocol. Using morphological observation, immunocytochemistry, gene expression and flow cytometry, it was shown predominantly multiple lineages of neuroectodermal cells were formed in our protocol and to a lower extent, endodermal and mesodermal differentiated cell types. This abbreviated protocol should lead to an advanced screening method using morphology in combination with selected differentiation markers aimed at predicting neurodevelopmental toxicity. Finally, the assay was shown to express differential sensitivity to a model developmental neurotoxicant, methyl mercury." @default.
- W2025366145 created "2016-06-24" @default.
- W2025366145 creator A5006930262 @default.
- W2025366145 creator A5010620767 @default.
- W2025366145 creator A5030430307 @default.
- W2025366145 creator A5038697595 @default.
- W2025366145 creator A5049906856 @default.
- W2025366145 creator A5073820178 @default.
- W2025366145 date "2010-07-01" @default.
- W2025366145 modified "2023-10-11" @default.
- W2025366145 title "An abbreviated protocol for multilineage neural differentiation of murine embryonic stem cells and its perturbation by methyl mercury" @default.
- W2025366145 cites W1485734931 @default.
- W2025366145 cites W1505732106 @default.
- W2025366145 cites W1551925364 @default.
- W2025366145 cites W1652680663 @default.
- W2025366145 cites W1746095826 @default.
- W2025366145 cites W1966109513 @default.
- W2025366145 cites W1970342920 @default.
- W2025366145 cites W1974243054 @default.
- W2025366145 cites W1976272679 @default.
- W2025366145 cites W1976463319 @default.
- W2025366145 cites W1982555416 @default.
- W2025366145 cites W1983879760 @default.
- W2025366145 cites W1998641354 @default.
- W2025366145 cites W2007648279 @default.
- W2025366145 cites W2016985320 @default.
- W2025366145 cites W2018576843 @default.
- W2025366145 cites W2019156031 @default.
- W2025366145 cites W2027246053 @default.
- W2025366145 cites W2051635348 @default.
- W2025366145 cites W2053702504 @default.
- W2025366145 cites W2056587633 @default.
- W2025366145 cites W2068841903 @default.
- W2025366145 cites W2076600697 @default.
- W2025366145 cites W2080199804 @default.
- W2025366145 cites W2085321399 @default.
- W2025366145 cites W2085457560 @default.
- W2025366145 cites W2086486740 @default.
- W2025366145 cites W2092246706 @default.
- W2025366145 cites W2093368594 @default.
- W2025366145 cites W2093452639 @default.
- W2025366145 cites W2100012145 @default.
- W2025366145 cites W2115346246 @default.
- W2025366145 cites W2117715664 @default.
- W2025366145 cites W2119583074 @default.
- W2025366145 cites W2129957319 @default.
- W2025366145 cites W2142283610 @default.
- W2025366145 cites W2147659971 @default.
- W2025366145 cites W2156522297 @default.
- W2025366145 cites W2161687343 @default.
- W2025366145 cites W2170713118 @default.
- W2025366145 cites W2172692677 @default.
- W2025366145 cites W2341491220 @default.
- W2025366145 cites W27814405 @default.
- W2025366145 doi "https://doi.org/10.1016/j.reprotox.2010.04.003" @default.
- W2025366145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20412851" @default.
- W2025366145 hasPublicationYear "2010" @default.
- W2025366145 type Work @default.
- W2025366145 sameAs 2025366145 @default.
- W2025366145 citedByCount "69" @default.
- W2025366145 countsByYear W20253661452012 @default.
- W2025366145 countsByYear W20253661452013 @default.
- W2025366145 countsByYear W20253661452014 @default.
- W2025366145 countsByYear W20253661452015 @default.
- W2025366145 countsByYear W20253661452016 @default.
- W2025366145 countsByYear W20253661452017 @default.
- W2025366145 countsByYear W20253661452018 @default.
- W2025366145 countsByYear W20253661452019 @default.
- W2025366145 countsByYear W20253661452020 @default.
- W2025366145 countsByYear W20253661452021 @default.
- W2025366145 countsByYear W20253661452022 @default.
- W2025366145 countsByYear W20253661452023 @default.
- W2025366145 crossrefType "journal-article" @default.
- W2025366145 hasAuthorship W2025366145A5006930262 @default.
- W2025366145 hasAuthorship W2025366145A5010620767 @default.
- W2025366145 hasAuthorship W2025366145A5030430307 @default.
- W2025366145 hasAuthorship W2025366145A5038697595 @default.
- W2025366145 hasAuthorship W2025366145A5049906856 @default.
- W2025366145 hasAuthorship W2025366145A5073820178 @default.
- W2025366145 hasConcept C104317684 @default.
- W2025366145 hasConcept C126322002 @default.
- W2025366145 hasConcept C134018914 @default.
- W2025366145 hasConcept C136834591 @default.
- W2025366145 hasConcept C145103041 @default.
- W2025366145 hasConcept C148738053 @default.
- W2025366145 hasConcept C153911025 @default.
- W2025366145 hasConcept C179335377 @default.
- W2025366145 hasConcept C2779234561 @default.
- W2025366145 hasConcept C28328180 @default.
- W2025366145 hasConcept C29730261 @default.
- W2025366145 hasConcept C35866371 @default.
- W2025366145 hasConcept C461258 @default.
- W2025366145 hasConcept C46973012 @default.
- W2025366145 hasConcept C54355233 @default.
- W2025366145 hasConcept C553184892 @default.
- W2025366145 hasConcept C71924100 @default.
- W2025366145 hasConcept C86803240 @default.
- W2025366145 hasConcept C95444343 @default.