Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025375204> ?p ?o ?g. }
- W2025375204 endingPage "2085" @default.
- W2025375204 startingPage "2078" @default.
- W2025375204 abstract "Two alkyl alcohol dehydrogenase (ADH) genes from the long-chain alkane-degrading strain Geobacillus thermodenitrificans NG80-2 were characterized in vitro . ADH1 and ADH2 were prepared heterologously in Escherichia coli as a homooctameric and a homodimeric protein, respectively. Both ADHs can oxidize a broad range of alkyl alcohols up to at least C 30 , as well as 1,3-propanediol and acetaldehyde. ADH1 also oxidizes glycerol, and ADH2 oxidizes isopropyl alcohol, isoamylol, acetone, octanal and decanal. The best substrate is ethanol for ADH1 and 1-octanol for ADH2. For both ADHs, the optimum assay condition is at 60 °C and pH 8.0, and both NAD and NADP can be used as the cofactor. Sequence analysis reveals that ADH1 and ADH2 belong to the Fe-containing/activated long-chain ADHs. However, the two enzymes contain neither Fe nor other metals, and Fe is not required for the activity, suggesting a new type of ADH. The ADHs characterized here are potentially useful in crude oil bioremediation and other bioconversion processes." @default.
- W2025375204 created "2016-06-24" @default.
- W2025375204 creator A5015756280 @default.
- W2025375204 creator A5016049539 @default.
- W2025375204 creator A5018714664 @default.
- W2025375204 creator A5038351828 @default.
- W2025375204 creator A5059594394 @default.
- W2025375204 creator A5073216396 @default.
- W2025375204 date "2009-06-01" @default.
- W2025375204 modified "2023-09-26" @default.
- W2025375204 title "Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2" @default.
- W2025375204 cites W1492708543 @default.
- W2025375204 cites W1546729927 @default.
- W2025375204 cites W1551631513 @default.
- W2025375204 cites W1554218007 @default.
- W2025375204 cites W1569378862 @default.
- W2025375204 cites W1602205078 @default.
- W2025375204 cites W1603897681 @default.
- W2025375204 cites W1783650594 @default.
- W2025375204 cites W1869531661 @default.
- W2025375204 cites W1965180905 @default.
- W2025375204 cites W1977384337 @default.
- W2025375204 cites W1977656136 @default.
- W2025375204 cites W1984198156 @default.
- W2025375204 cites W1997894058 @default.
- W2025375204 cites W2002644070 @default.
- W2025375204 cites W2008237642 @default.
- W2025375204 cites W2041327722 @default.
- W2025375204 cites W2064626494 @default.
- W2025375204 cites W2067542599 @default.
- W2025375204 cites W2070769048 @default.
- W2025375204 cites W2073553628 @default.
- W2025375204 cites W2079640524 @default.
- W2025375204 cites W2084820795 @default.
- W2025375204 cites W2099542473 @default.
- W2025375204 cites W2100498403 @default.
- W2025375204 cites W2100837269 @default.
- W2025375204 cites W2104346648 @default.
- W2025375204 cites W2107336582 @default.
- W2025375204 cites W2116050866 @default.
- W2025375204 cites W2117842859 @default.
- W2025375204 cites W2119729375 @default.
- W2025375204 cites W2123949437 @default.
- W2025375204 cites W2131610468 @default.
- W2025375204 cites W2134037455 @default.
- W2025375204 cites W2141097001 @default.
- W2025375204 cites W2144376029 @default.
- W2025375204 cites W2145236813 @default.
- W2025375204 cites W2156899368 @default.
- W2025375204 cites W4293247451 @default.
- W2025375204 doi "https://doi.org/10.1099/mic.0.027201-0" @default.
- W2025375204 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19383697" @default.
- W2025375204 hasPublicationYear "2009" @default.
- W2025375204 type Work @default.
- W2025375204 sameAs 2025375204 @default.
- W2025375204 citedByCount "27" @default.
- W2025375204 countsByYear W20253752042012 @default.
- W2025375204 countsByYear W20253752042013 @default.
- W2025375204 countsByYear W20253752042014 @default.
- W2025375204 countsByYear W20253752042016 @default.
- W2025375204 countsByYear W20253752042017 @default.
- W2025375204 countsByYear W20253752042018 @default.
- W2025375204 countsByYear W20253752042019 @default.
- W2025375204 countsByYear W20253752042021 @default.
- W2025375204 countsByYear W20253752042022 @default.
- W2025375204 countsByYear W20253752042023 @default.
- W2025375204 crossrefType "journal-article" @default.
- W2025375204 hasAuthorship W2025375204A5015756280 @default.
- W2025375204 hasAuthorship W2025375204A5016049539 @default.
- W2025375204 hasAuthorship W2025375204A5018714664 @default.
- W2025375204 hasAuthorship W2025375204A5038351828 @default.
- W2025375204 hasAuthorship W2025375204A5059594394 @default.
- W2025375204 hasAuthorship W2025375204A5073216396 @default.
- W2025375204 hasConcept C178790620 @default.
- W2025375204 hasConcept C181199279 @default.
- W2025375204 hasConcept C185592680 @default.
- W2025375204 hasConcept C197957613 @default.
- W2025375204 hasConcept C2776317432 @default.
- W2025375204 hasConcept C2777344606 @default.
- W2025375204 hasConcept C2780263894 @default.
- W2025375204 hasConcept C2780437130 @default.
- W2025375204 hasConcept C2781066024 @default.
- W2025375204 hasConcept C2781415988 @default.
- W2025375204 hasConcept C55493867 @default.
- W2025375204 hasConcept C71240020 @default.
- W2025375204 hasConcept C75520062 @default.
- W2025375204 hasConceptScore W2025375204C178790620 @default.
- W2025375204 hasConceptScore W2025375204C181199279 @default.
- W2025375204 hasConceptScore W2025375204C185592680 @default.
- W2025375204 hasConceptScore W2025375204C197957613 @default.
- W2025375204 hasConceptScore W2025375204C2776317432 @default.
- W2025375204 hasConceptScore W2025375204C2777344606 @default.
- W2025375204 hasConceptScore W2025375204C2780263894 @default.
- W2025375204 hasConceptScore W2025375204C2780437130 @default.
- W2025375204 hasConceptScore W2025375204C2781066024 @default.
- W2025375204 hasConceptScore W2025375204C2781415988 @default.
- W2025375204 hasConceptScore W2025375204C55493867 @default.
- W2025375204 hasConceptScore W2025375204C71240020 @default.