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- W2025485612 abstract "The genes encoding the endonuclease and the methylase of the Pvu l restriction and modification system were cloned in E.coli and characterized. The genes were adjacent in tandem orientation spanning a distance of 2200 bases. The Pvu l endonuclease was a single polypeptide with a calculated molecular weight of 27,950 daltons. The endonuclease was easily detectable when the gene was expressed from its endogenous promotor and present on a low copy plasmid, but expression was considerably enhanced when the endonuclease gene was placed under the control of a strong promotor on a high copy plasmid. The methylase did not completely protect plasmid DNA from R· Pvu l digestion until the methylase gene was placed under lac promotor control in a multicopy plasmid. In the absence of the M· Pvu l methylase, expression of the R· Pvu l endonuclease from the lac promotor on a multicopy plasmid was not lethal to wild type E.coli , but was lethal in a temperature-sensitive ligase mutant at the non-permissive temperature. Moreover, induction of the R· Pvu l endonuclease under λpL promotor control resulted in complete digestion of the E.coli chromosome by R· Pvu l." @default.
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- W2025485612 title "Cloning and characterization of genes for thePvul restriction and modification system" @default.
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- W2025485612 doi "https://doi.org/10.1093/nar/20.21.5743" @default.
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