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• W2025486571 startingPage "279" @default.
• W2025486571 abstract "The instability of cell cultures containing plasmid vectors is a major problem in the commercial exploitation of molecular cloning techniques. Plasmid stability is influenced by the nature of the host cell, the type of plasmid and/or environmental conditions. Plasmid encoded properties may confer a selective advantage on the host cell but can be an energy drain due to replication and expression. Stability of recombinant cultures ultimately may be determined by the cost to benefit ratio of plasmid carriage. The relative competition between plasmid containing and plasmid-free or indigenous populations can determine the degree of dominance of recombinant cultures. The use of inocula in biotechnological processes in which dynamic environmental conditions dominate may also result in instabilities resulting from the characteristics of the ecosystem. In such dynamic conditions plasmid stability is just one contribution to culture stability. Strategies to enhance plasmid stability, within such environments, based on manipulation of physiological state of host cells, must consider the responsiveness or plasticity of both cells and populations. The robustness of cells or the responses to stresses or transient environmental conditions can influence the levels of instability detected; for example, instability or mutation in the host genome may lead to enhanced plasmid stability. Competition among subpopulations arising from unstable copy number control may determine the levels of recombinant cells in open versus closed fermenter systems. Thus the ecological competence (ability to survive and compete) of recombinant cells in dynamic or transient environments is fundamental to the understanding of the ultimate dominance or survival of such recombinant cultures and may form the basis of a strategy to enhance or control stability either in fermenter systems or dynamic process environments. The creation of microniches in time and/or space can enhance plasmid stability. Transient operation based on defined environmental stresses or perturbations in fermenter systems or in heterogeneous or dynamic environments found in gel immobilized cultures have resulted in enhanced stability. Spatial organization resulting from immobilization has the additional advantage of regulated cell protection within defined microenvironments and controlled release, depending on the nature of the gel, from these microenvironments or microcosms. This regulation of ecological competence allied to the advantages of microbial cell growth in gel microenvironments combined with the spatial organization (or juxtapositioning of cells, selective agents, nutrients, protectants, etc.) possible through immobilization technology offers new strategies to enhance plasmid and culture stability." @default.
• W2025486571 created "2016-06-24" @default.
• W2025486571 creator A5034581499 @default.
• W2025486571 date "1994-01-01" @default.
• W2025486571 modified "2023-10-18" @default.
• W2025486571 title "Plasmid stability and ecological competence in recombinant cultures" @default.
• W2025486571 cites W12365609 @default.
• W2025486571 cites W1487247933 @default.
• W2025486571 cites W1487581209 @default.
• W2025486571 cites W1494498965 @default.
• W2025486571 cites W1513386749 @default.
• W2025486571 cites W1516194903 @default.
• W2025486571 cites W1536934635 @default.
• W2025486571 cites W1557830462 @default.
• W2025486571 cites W1560038937 @default.
• W2025486571 cites W1561950983 @default.
• W2025486571 cites W1566350652 @default.
• W2025486571 cites W1566957489 @default.
• W2025486571 cites W1568688238 @default.
• W2025486571 cites W1584328291 @default.
• W2025486571 cites W1587672105 @default.
• W2025486571 cites W1596351537 @default.
• W2025486571 cites W1596526854 @default.
• W2025486571 cites W1597072824 @default.
• W2025486571 cites W1597810131 @default.
• W2025486571 cites W1598986755 @default.
• W2025486571 cites W1599170228 @default.
• W2025486571 cites W1601991546 @default.
• W2025486571 cites W1643763588 @default.
• W2025486571 cites W1660195041 @default.
• W2025486571 cites W1667731728 @default.
• W2025486571 cites W1784740672 @default.
• W2025486571 cites W1804665837 @default.
• W2025486571 cites W1815303570 @default.
• W2025486571 cites W1897676431 @default.
• W2025486571 cites W1923326679 @default.
• W2025486571 cites W1923732320 @default.
• W2025486571 cites W1960533781 @default.
• W2025486571 cites W1963812440 @default.
• W2025486571 cites W1964343264 @default.
• W2025486571 cites W1966849744 @default.
• W2025486571 cites W1967185707 @default.
• W2025486571 cites W1970660972 @default.
• W2025486571 cites W1973260146 @default.
• W2025486571 cites W1973670099 @default.
• W2025486571 cites W1974470327 @default.
• W2025486571 cites W1974631048 @default.
• W2025486571 cites W1977845046 @default.
• W2025486571 cites W1978009343 @default.
• W2025486571 cites W1978743625 @default.
• W2025486571 cites W1979438017 @default.
• W2025486571 cites W1980116860 @default.
• W2025486571 cites W1981369765 @default.
• W2025486571 cites W1981802246 @default.
• W2025486571 cites W1982468347 @default.
• W2025486571 cites W1983719666 @default.
• W2025486571 cites W1983891308 @default.
• W2025486571 cites W1984061267 @default.
• W2025486571 cites W1985085715 @default.
• W2025486571 cites W1989700291 @default.
• W2025486571 cites W1989974224 @default.
• W2025486571 cites W1991177095 @default.
• W2025486571 cites W1992058103 @default.
• W2025486571 cites W1993371455 @default.
• W2025486571 cites W1995025557 @default.
• W2025486571 cites W1996304242 @default.
• W2025486571 cites W1996528295 @default.
• W2025486571 cites W1997491947 @default.
• W2025486571 cites W1999115523 @default.
• W2025486571 cites W2001452066 @default.
• W2025486571 cites W2006503784 @default.
• W2025486571 cites W2006690055 @default.
• W2025486571 cites W2006783637 @default.
• W2025486571 cites W2008497514 @default.
• W2025486571 cites W2008752334 @default.
• W2025486571 cites W2008897847 @default.
• W2025486571 cites W2009465304 @default.
• W2025486571 cites W2010781821 @default.
• W2025486571 cites W2010885966 @default.
• W2025486571 cites W2014496483 @default.
• W2025486571 cites W2014637200 @default.
• W2025486571 cites W2015236470 @default.
• W2025486571 cites W2015763180 @default.
• W2025486571 cites W2015864763 @default.
• W2025486571 cites W2022381862 @default.
• W2025486571 cites W2022965248 @default.
• W2025486571 cites W2023171502 @default.
• W2025486571 cites W2023364466 @default.
• W2025486571 cites W2026504501 @default.
• W2025486571 cites W2027831635 @default.
• W2025486571 cites W2028756649 @default.
• W2025486571 cites W2030036361 @default.
• W2025486571 cites W2034653975 @default.
• W2025486571 cites W2034676264 @default.
• W2025486571 cites W2035301827 @default.
• W2025486571 cites W2035667664 @default.
• W2025486571 cites W2035709881 @default.
• W2025486571 cites W2040004887 @default.

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