Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025540178> ?p ?o ?g. }
- W2025540178 endingPage "89" @default.
- W2025540178 startingPage "79" @default.
- W2025540178 abstract "Conditions for the rigorous purification of desulfoviridin, the dissimilatory sulfite reductase from the sulfate-reducing bacterium Desulfovibrio vulgaris (Hildenborough) have been established. A final purification by fast protein liquid chromatography yields at least three distinct bands that each exhibit the characteristic absorption spectrum of desulfoviridin. Two of these have been extensively characterized by amino acid analysis, isoelectric focusing, polyacrylamide gel electrophoresis, and formulation of the prosthetic centers. Each contains two pairs of [Fe4S4] and siroheme units. These results stand in marked contrast to recent work claiming significant demetallation of siroheme, excess iron content, and the presence of Fe6S6 clusters. These proposals are critically assessed in light of our results and other published work. Steady-state kinetic parameters have been determined: kcat(SO32-) = 0.31 mol SO32-· s−1· mol heme−1, Km= 0.06 mM; kcat(NO2−) = 0.038 mol NO2−· s−1· mol heme−1, Km= 0.028 mM; kcat(NH2OH) = 29 mol NH2OH · s−1· mol heme−1, Km= 48 mM. A detailed comparison is made with the Escherichia coli and spinach-assimilatory sulfite reductase enzymes and spinach nitrite reductase. Highly purified samples of dissimilatory sulfite reductase display an electron paramagnetic resonance spectrum characteristic of rhombic high spin ferric heme centers, while the fully reduced enzyme shows EPR features typical of [Fe4S4] clusters. The magnetic properties of the prosthetic centers are further characterized by variable temperature experiments and spin quantitation." @default.
- W2025540178 created "2016-06-24" @default.
- W2025540178 creator A5011309627 @default.
- W2025540178 creator A5056809807 @default.
- W2025540178 creator A5085833582 @default.
- W2025540178 date "1994-07-01" @default.
- W2025540178 modified "2023-10-03" @default.
- W2025540178 title "Desulfoviridin, a multimeric-dissimilatory sulfite reductase from Desulfovibrio vulgaris (Hildenborough) Purification, characterization, kinetics and EPR studies" @default.
- W2025540178 cites W1158350440 @default.
- W2025540178 cites W1472228319 @default.
- W2025540178 cites W1485123151 @default.
- W2025540178 cites W1489679748 @default.
- W2025540178 cites W1518359924 @default.
- W2025540178 cites W1538196126 @default.
- W2025540178 cites W1549951942 @default.
- W2025540178 cites W1568462943 @default.
- W2025540178 cites W1575516480 @default.
- W2025540178 cites W1584799712 @default.
- W2025540178 cites W1588237320 @default.
- W2025540178 cites W1589586705 @default.
- W2025540178 cites W1602084887 @default.
- W2025540178 cites W1880548776 @default.
- W2025540178 cites W1911499593 @default.
- W2025540178 cites W1970331184 @default.
- W2025540178 cites W1971101514 @default.
- W2025540178 cites W1971303670 @default.
- W2025540178 cites W1974803825 @default.
- W2025540178 cites W1996811886 @default.
- W2025540178 cites W2006694214 @default.
- W2025540178 cites W2006924060 @default.
- W2025540178 cites W2008363621 @default.
- W2025540178 cites W2008557757 @default.
- W2025540178 cites W2018723916 @default.
- W2025540178 cites W2021037958 @default.
- W2025540178 cites W2025060094 @default.
- W2025540178 cites W2040145161 @default.
- W2025540178 cites W2040453384 @default.
- W2025540178 cites W2048882269 @default.
- W2025540178 cites W2051236807 @default.
- W2025540178 cites W2057533555 @default.
- W2025540178 cites W2060121377 @default.
- W2025540178 cites W2060703713 @default.
- W2025540178 cites W2065314019 @default.
- W2025540178 cites W2065477455 @default.
- W2025540178 cites W2077154171 @default.
- W2025540178 cites W2083596866 @default.
- W2025540178 cites W2085153350 @default.
- W2025540178 cites W2094618408 @default.
- W2025540178 cites W2103654377 @default.
- W2025540178 cites W2229118857 @default.
- W2025540178 cites W2318013630 @default.
- W2025540178 cites W2414317236 @default.
- W2025540178 cites W2424412083 @default.
- W2025540178 cites W2460647633 @default.
- W2025540178 cites W2892850364 @default.
- W2025540178 cites W959024556 @default.
- W2025540178 doi "https://doi.org/10.1111/j.1432-1033.1994.tb18968.x" @default.
- W2025540178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8033912" @default.
- W2025540178 hasPublicationYear "1994" @default.
- W2025540178 type Work @default.
- W2025540178 sameAs 2025540178 @default.
- W2025540178 citedByCount "67" @default.
- W2025540178 countsByYear W20255401782012 @default.
- W2025540178 countsByYear W20255401782013 @default.
- W2025540178 countsByYear W20255401782014 @default.
- W2025540178 countsByYear W20255401782015 @default.
- W2025540178 countsByYear W20255401782016 @default.
- W2025540178 countsByYear W20255401782017 @default.
- W2025540178 countsByYear W20255401782018 @default.
- W2025540178 countsByYear W20255401782019 @default.
- W2025540178 countsByYear W20255401782021 @default.
- W2025540178 countsByYear W20255401782022 @default.
- W2025540178 countsByYear W20255401782023 @default.
- W2025540178 crossrefType "journal-article" @default.
- W2025540178 hasAuthorship W2025540178A5011309627 @default.
- W2025540178 hasAuthorship W2025540178A5056809807 @default.
- W2025540178 hasAuthorship W2025540178A5085833582 @default.
- W2025540178 hasConcept C121332964 @default.
- W2025540178 hasConcept C134651460 @default.
- W2025540178 hasConcept C178790620 @default.
- W2025540178 hasConcept C179104552 @default.
- W2025540178 hasConcept C181199279 @default.
- W2025540178 hasConcept C185592680 @default.
- W2025540178 hasConcept C187961010 @default.
- W2025540178 hasConcept C2776217839 @default.
- W2025540178 hasConcept C2777107655 @default.
- W2025540178 hasConcept C2777403493 @default.
- W2025540178 hasConcept C2778343803 @default.
- W2025540178 hasConcept C2778710519 @default.
- W2025540178 hasConcept C2779830873 @default.
- W2025540178 hasConcept C2780534640 @default.
- W2025540178 hasConcept C2781243026 @default.
- W2025540178 hasConcept C2781341435 @default.
- W2025540178 hasConcept C41183919 @default.
- W2025540178 hasConcept C46141821 @default.
- W2025540178 hasConcept C523546767 @default.
- W2025540178 hasConcept C54355233 @default.
- W2025540178 hasConcept C55493867 @default.