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- W2025552622 abstract "A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme degrades type IV collagen, but neither type I collagen nor casein. The failure of trypsin treatment to enhance the enzyme activity suggested that the purified enzyme did not require activation. Although the enzyme seems to be classified as a matrix metalloproteinase, it was inhibited by neither tissue inhibitor of metalloproteinases (TIMP) nor TIMP-2 and thus represents a novel type IV collagen-degrading metalloproteinase." @default.
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- W2025552622 date "1990-07-30" @default.
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- W2025552622 title "A novel TIMP-insensitive type IV collagen-degrading metalloproteinase from murine metastatic sarcoma cells" @default.
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- W2025552622 doi "https://doi.org/10.1016/0014-5793(90)80967-n" @default.
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