Matches in SemOpenAlex for { <https://semopenalex.org/work/W202556135> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W202556135 endingPage "1021" @default.
- W202556135 startingPage "1021" @default.
- W202556135 abstract "1021 Objectives This study aims to develop reporter gene targeting method for AFP-producing hepatocellular carcinoma (HCC) specific imaging and therapy using adenovirus vector containing reporter gene driven by AFP enhancer/promoter. Methods The recombinant adenovirus vectors, AdAFPfLuc and AdAFPhNIS (containing firefly luciferase gene and human sodium/iodide symporter gene, respectively, driven by human AFP enhancer/promoter) were prepared. After an in vitro infection by adenovirus, the expression of reporter genes was confirmed by luciferase assay, I-125 uptake assay and RT-PCR analysis in AFP-producing cells (HuH-7 and HepG2), and in AFP-nonproducing cells (SK-Hep-1, Chang, and C6). The tumor-bearing mice were intravenously injected with adenovirus, and bioluminescent and scintigraphic images were obtained. Results The expression of fLuc or hNIS was demonstrated efficiently by luciferase assay or I-125 uptake assay in AFP-producing cells, but not in AFP-nonproducing cells. Tumor specific reporter gene expression was confirmed on mRNA level by RT-PCR analysis. Injected intravenously in HuH-7 tumor bearing mice, adenovirus drove expression of a functional fLuc or hNIS protein by only HuH-7 tumor and allowed marked luciferase or hNIS activity, whereas AdCMVLuc drove fLuc expression only in liver. Conclusions AFP-producing HCC was targeted with adenovirus vector containing reporter gene using AFP enhancer/promoter in vitro and in vivo. These findings demonstrate that AFP-producing HCC specific molecular imaging are feasible using this recombinant adenovirus vector system." @default.
- W202556135 created "2016-06-24" @default.
- W202556135 creator A5012860746 @default.
- W202556135 creator A5016774173 @default.
- W202556135 creator A5019507448 @default.
- W202556135 creator A5039890801 @default.
- W202556135 creator A5046877593 @default.
- W202556135 creator A5050782603 @default.
- W202556135 creator A5066151567 @default.
- W202556135 creator A5074199688 @default.
- W202556135 creator A5089054890 @default.
- W202556135 creator A5091362044 @default.
- W202556135 date "2009-05-01" @default.
- W202556135 modified "2023-10-15" @default.
- W202556135 title "Hepatocellular carcinoma targeted reporter gene expression using alpha-fetoprotein (AFP) enhancer/promoter in mouse model" @default.
- W202556135 hasPublicationYear "2009" @default.
- W202556135 type Work @default.
- W202556135 sameAs 202556135 @default.
- W202556135 citedByCount "0" @default.
- W202556135 crossrefType "journal-article" @default.
- W202556135 hasAuthorship W202556135A5012860746 @default.
- W202556135 hasAuthorship W202556135A5016774173 @default.
- W202556135 hasAuthorship W202556135A5019507448 @default.
- W202556135 hasAuthorship W202556135A5039890801 @default.
- W202556135 hasAuthorship W202556135A5046877593 @default.
- W202556135 hasAuthorship W202556135A5050782603 @default.
- W202556135 hasAuthorship W202556135A5066151567 @default.
- W202556135 hasAuthorship W202556135A5074199688 @default.
- W202556135 hasAuthorship W202556135A5089054890 @default.
- W202556135 hasAuthorship W202556135A5091362044 @default.
- W202556135 hasConcept C104317684 @default.
- W202556135 hasConcept C111335760 @default.
- W202556135 hasConcept C111599444 @default.
- W202556135 hasConcept C111936080 @default.
- W202556135 hasConcept C120405084 @default.
- W202556135 hasConcept C149011108 @default.
- W202556135 hasConcept C150194340 @default.
- W202556135 hasConcept C153911025 @default.
- W202556135 hasConcept C161733203 @default.
- W202556135 hasConcept C2778019345 @default.
- W202556135 hasConcept C2780616701 @default.
- W202556135 hasConcept C32470452 @default.
- W202556135 hasConcept C40767141 @default.
- W202556135 hasConcept C502942594 @default.
- W202556135 hasConcept C54009773 @default.
- W202556135 hasConcept C55493867 @default.
- W202556135 hasConcept C86803240 @default.
- W202556135 hasConceptScore W202556135C104317684 @default.
- W202556135 hasConceptScore W202556135C111335760 @default.
- W202556135 hasConceptScore W202556135C111599444 @default.
- W202556135 hasConceptScore W202556135C111936080 @default.
- W202556135 hasConceptScore W202556135C120405084 @default.
- W202556135 hasConceptScore W202556135C149011108 @default.
- W202556135 hasConceptScore W202556135C150194340 @default.
- W202556135 hasConceptScore W202556135C153911025 @default.
- W202556135 hasConceptScore W202556135C161733203 @default.
- W202556135 hasConceptScore W202556135C2778019345 @default.
- W202556135 hasConceptScore W202556135C2780616701 @default.
- W202556135 hasConceptScore W202556135C32470452 @default.
- W202556135 hasConceptScore W202556135C40767141 @default.
- W202556135 hasConceptScore W202556135C502942594 @default.
- W202556135 hasConceptScore W202556135C54009773 @default.
- W202556135 hasConceptScore W202556135C55493867 @default.
- W202556135 hasConceptScore W202556135C86803240 @default.
- W202556135 hasLocation W2025561351 @default.
- W202556135 hasOpenAccess W202556135 @default.
- W202556135 hasPrimaryLocation W2025561351 @default.
- W202556135 hasRelatedWork W1569585729 @default.
- W202556135 hasRelatedWork W2239658161 @default.
- W202556135 hasRelatedWork W2338016524 @default.
- W202556135 hasRelatedWork W2347905088 @default.
- W202556135 hasRelatedWork W2349844359 @default.
- W202556135 hasRelatedWork W2350700060 @default.
- W202556135 hasRelatedWork W2356402407 @default.
- W202556135 hasRelatedWork W2372331831 @default.
- W202556135 hasRelatedWork W2376494660 @default.
- W202556135 hasRelatedWork W2381343262 @default.
- W202556135 hasRelatedWork W2381683784 @default.
- W202556135 hasRelatedWork W2388423011 @default.
- W202556135 hasRelatedWork W2390076375 @default.
- W202556135 hasRelatedWork W2393717075 @default.
- W202556135 hasRelatedWork W2406318196 @default.
- W202556135 hasRelatedWork W2420611152 @default.
- W202556135 hasRelatedWork W3031040604 @default.
- W202556135 hasRelatedWork W3032440438 @default.
- W202556135 hasRelatedWork W3163459669 @default.
- W202556135 hasRelatedWork W875510739 @default.
- W202556135 hasVolume "50" @default.
- W202556135 isParatext "false" @default.
- W202556135 isRetracted "false" @default.
- W202556135 magId "202556135" @default.
- W202556135 workType "article" @default.