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- W2025561572 abstract "Abstract Media were compared for culture of the estuarine plant Ruppia maritima L. The best inorganic nutrient amendment was based on f/2 basal enrichment salts in artificial seawater. Using f/2 basal enrichments, Ruppia maritima could be grown in media where either bicarbonate or sucrose was provided as the major carbon source. The cytokinin 2iP (6[γ,γ-dimethylallylamino]-purine) stimulated more growth than BA (N6-benzylaminopurine) in sucrose-based cultures. There was no significant effect of cytokinin addition in cultures growing in a bicarbonate-based medium. The addition of five vitamins did not improve growth. In an f/2 medium with bicarbonate as the carbon source, a pH of 7.5 appears to be adequate. Use of f/2 nutrient salts resulted in more growth than f/4 nutrients. These data suggest that the seagrass R. maritima grows well in a completely inorganic medium. Ruppia maritima appears to show physiological characteristics of an opportunistic species: minimal nutritional requirements and no requirements for complex exogenous growth substances. Cultures of this seagrass would be useful for a number of physiological, biochemical and environmental restoration studies." @default.
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- W2025561572 date "1996-08-01" @default.
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- W2025561572 title "Culture studies of Ruppia maritima L. in bicarbonate- and sucrose-based media" @default.
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- W2025561572 doi "https://doi.org/10.1016/0022-0981(95)00195-6" @default.
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