Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025585440> ?p ?o ?g. }
- W2025585440 endingPage "2923" @default.
- W2025585440 startingPage "2908" @default.
- W2025585440 abstract "CRH-binding protein (CRH-BP) regulates activation of the hypothalamic-pituitary-adrenal (HPA) axis by binding and inhibiting CRH. We investigated for the first time transcriptional regulation of the human CRH-BP promoter using transient transfections. Estrogen receptors (ERs) contributed to ligand-independent constitutive activation of the promoter, whereas in the presence of estradiol ERα induced and ERβ repressed promoter activity in a dose-dependent manner. TNFα inhibited promoter induction by ERα in the absence and presence of estradiol. Three ERE half-sites in the CRH-BP promoter bound ERα and ERβ in an EMSA, and disruption of ERE half-sites by site-directed mutagenesis abolished ligand-independent induction by ERα and ERβ and promoter enhancement by estradiol-activated ERα. Repression by estradiol/ERβ was unaffected by disruption of ERE half-sites, activating protein 1, cAMP response element, GATA, or nuclear factor κB sites, and reversed to promoter induction by estrogen antagonists, tamoxifen and ICI 182,780, suggesting corepressor involvement. In hypothalamic GT1–7 cells, Western blotting demonstrated rapid induction of endogenous CRH-BP expression by estradiol-bound ER, which was inhibited by TNFα. We propose a model in which ERs maintain basal CRH-BP expression in pituitary and neurosecretory cells, whereas in the presence of ERα estrogen enhances CRH-BP transcription, causing down-regulation of the HPA axis, and nuclear factor κB-activating cytokines activate the HPA axis by inhibiting ERα." @default.
- W2025585440 created "2016-06-24" @default.
- W2025585440 creator A5006582605 @default.
- W2025585440 creator A5008671687 @default.
- W2025585440 creator A5019893954 @default.
- W2025585440 creator A5026500855 @default.
- W2025585440 creator A5027062796 @default.
- W2025585440 creator A5045722436 @default.
- W2025585440 creator A5070874607 @default.
- W2025585440 creator A5080749375 @default.
- W2025585440 date "2004-12-01" @default.
- W2025585440 modified "2023-10-02" @default.
- W2025585440 title "Estrogen Receptor (ER)-Mediated Transcriptional Regulation of the Human Corticotropin-Releasing Hormone-Binding Protein Promoter: Differential Effects of ERα and ERβ" @default.
- W2025585440 cites W1590197184 @default.
- W2025585440 cites W1659749843 @default.
- W2025585440 cites W1736447480 @default.
- W2025585440 cites W1787106071 @default.
- W2025585440 cites W1908878855 @default.
- W2025585440 cites W1970755773 @default.
- W2025585440 cites W1971767029 @default.
- W2025585440 cites W1976382352 @default.
- W2025585440 cites W1988871294 @default.
- W2025585440 cites W1989455449 @default.
- W2025585440 cites W1990529844 @default.
- W2025585440 cites W1991455388 @default.
- W2025585440 cites W1994673724 @default.
- W2025585440 cites W2004954728 @default.
- W2025585440 cites W2009558707 @default.
- W2025585440 cites W2010121586 @default.
- W2025585440 cites W2014537263 @default.
- W2025585440 cites W2018111331 @default.
- W2025585440 cites W2019175546 @default.
- W2025585440 cites W2023319900 @default.
- W2025585440 cites W2023344951 @default.
- W2025585440 cites W2026135238 @default.
- W2025585440 cites W2026443131 @default.
- W2025585440 cites W2027109061 @default.
- W2025585440 cites W2035415315 @default.
- W2025585440 cites W2041276801 @default.
- W2025585440 cites W2042860057 @default.
- W2025585440 cites W2043915483 @default.
- W2025585440 cites W2044252104 @default.
- W2025585440 cites W2048909403 @default.
- W2025585440 cites W2052803306 @default.
- W2025585440 cites W2055430675 @default.
- W2025585440 cites W2059329097 @default.
- W2025585440 cites W2059474567 @default.
- W2025585440 cites W2061025691 @default.
- W2025585440 cites W2065827234 @default.
- W2025585440 cites W2067178891 @default.
- W2025585440 cites W2068527957 @default.
- W2025585440 cites W2078761249 @default.
- W2025585440 cites W2080487349 @default.
- W2025585440 cites W2083836198 @default.
- W2025585440 cites W2085600503 @default.
- W2025585440 cites W2092829453 @default.
- W2025585440 cites W2093676604 @default.
- W2025585440 cites W2122457225 @default.
- W2025585440 cites W2124762006 @default.
- W2025585440 cites W2142149120 @default.
- W2025585440 cites W2148017807 @default.
- W2025585440 cites W2151454086 @default.
- W2025585440 cites W2152840134 @default.
- W2025585440 cites W2152885509 @default.
- W2025585440 cites W2154937055 @default.
- W2025585440 cites W2159674010 @default.
- W2025585440 cites W2216094174 @default.
- W2025585440 cites W2411684832 @default.
- W2025585440 cites W3151167657 @default.
- W2025585440 cites W994887766 @default.
- W2025585440 doi "https://doi.org/10.1210/me.2003-0446" @default.
- W2025585440 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15345745" @default.
- W2025585440 hasPublicationYear "2004" @default.
- W2025585440 type Work @default.
- W2025585440 sameAs 2025585440 @default.
- W2025585440 citedByCount "46" @default.
- W2025585440 countsByYear W20255854402012 @default.
- W2025585440 countsByYear W20255854402013 @default.
- W2025585440 countsByYear W20255854402015 @default.
- W2025585440 countsByYear W20255854402016 @default.
- W2025585440 countsByYear W20255854402017 @default.
- W2025585440 countsByYear W20255854402019 @default.
- W2025585440 countsByYear W20255854402020 @default.
- W2025585440 countsByYear W20255854402021 @default.
- W2025585440 countsByYear W20255854402022 @default.
- W2025585440 crossrefType "journal-article" @default.
- W2025585440 hasAuthorship W2025585440A5006582605 @default.
- W2025585440 hasAuthorship W2025585440A5008671687 @default.
- W2025585440 hasAuthorship W2025585440A5019893954 @default.
- W2025585440 hasAuthorship W2025585440A5026500855 @default.
- W2025585440 hasAuthorship W2025585440A5027062796 @default.
- W2025585440 hasAuthorship W2025585440A5045722436 @default.
- W2025585440 hasAuthorship W2025585440A5070874607 @default.
- W2025585440 hasAuthorship W2025585440A5080749375 @default.
- W2025585440 hasBestOaLocation W20255854401 @default.
- W2025585440 hasConcept C101762097 @default.
- W2025585440 hasConcept C104317684 @default.
- W2025585440 hasConcept C107824862 @default.