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• W2025655051 abstract "Chitosan (CS) nanofibers with a diameter of 150–200 nm were fabricated from a mixed chitosan/poly (vinyl alcohol) (PVA) solution by the electrospinning method. The nascent CS/PVA nanofibers were treated with 0.5 M NaOH solution to make stable CS nanofibers by removing PVA under aqueous conditions. Hen egg-white lysozyme was immobilized on electrospun CS nanofibers via cross-linked enzyme aggregates (CLEAs) and used for effective and continuous antibacterial applications. The maximum amount of lysozyme immobilized on the CS nanofibers was determined to be 62.3 mg/g of nanofibers under the optimum conditions. The immobilized lysozyme-CLEA retained more than 75.4% of its initial activity after 80 days of storage at room temperature, while the free lysozyme lost all of its activity under the same conditions. In addition, the immobilized lysozyme-CLEA retained more than 76% of its activity after 100 consecutive uses. Finally, the durability of the lysozyme-CLEA immobilized CS nanofibers showed bacteriostasis ratios of 82.4%, 79.8%, 83.4%, and 84.1% after 10 cycles against 4 pathogenic bacteria, viz. Staphylococcus aureus, Bacillus subtilis, Shigella flexneri, and Psedomonas aeruginosa, respectively. These results demonstrated that lysozyme-CLEA immobilized CS nanofibers could be used as a promising material for enhanced and continuous antibacterial applications." @default.
• W2025655051 created "2016-06-24" @default.
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• W2025655051 date "2013-03-01" @default.
• W2025655051 modified "2023-09-27" @default.
• W2025655051 title "Immobilization of lysozyme-CLEA onto electrospun chitosan nanofiber for effective antibacterial applications" @default.
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• W2025655051 doi "https://doi.org/10.1016/j.ijbiomac.2012.11.025" @default.
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• W2025655051 hasPublicationYear "2013" @default.
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