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- W2025673029 abstract "We examined amounts and types of plasminogen activator and plasminogen activator inhibitor produced by cultured bovine mammary epithelial cells. The MAC-T and two other mammary epithelial cell lines, MACT-UV1 and MACT-UV2 derived from the parental MAC-T cells by subcloning, were used as model systems. Cells were cultured in a medium free of serum and protein. Data showed that MACT-UV2 cells produced 6.2 and 17.2% more plasminogen activator than MACT-UV1 and parental MAC-T cells, respectively. Addition of amiloride, a specific urokinase-plasminogen activator inhibitor, dramatically decreased the activity in the culture medium of parental and subclonal lines, indicating that urokinase-plasminogen activator was present. Zymography revealed the presence of urokinase-plasminogen activator with an approximate molecular mass of 50,000 kDa in the culture medium of parental MAC-T cells. The culture medium of the subclonal lines contained urokinase-plasminogen activator and tissue-plasminogen activator with approximate molecular masses of 50,000 and 75,000 kDa, respectively. Complexes of both types of plasminogen activators with plasminogen activator-inhibitor-1 were detected in the culture medium of subclonal lines." @default.
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- W2025673029 date "1994-10-01" @default.
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- W2025673029 title "Production of Various Forms of Plasminogen Activator and Plasminogen Activator Inhibitor by Cultured Mammary Eplthelial Cells" @default.
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- W2025673029 doi "https://doi.org/10.3168/jds.s0022-0302(94)77236-2" @default.
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