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- W2025681718 abstract "Further characterizations of 20β-hydroxysteroid dehydrogenase (20β-HSD) present in liver microsomes of male rats were examined. A significant relationship was observed between 20β-HSD and acetohexamide reductase (AHR) activities in liver microsomes of male rats. The hepatic microsomal 20β-HSD and AHR preferentially required NADPH as a cofactor. When NADPH was replaced by NADH, NADP or NAD at the same concentration, these reductase activities were little detected. The hepatic microsomal 20β-HSD and AHR activities in streptozotocin-induced diabetic rats were much lower than those in the corresponding controls. The hepatic microsomal 20β-HSD and AHR activities appeared as one main peak, respectively, on DEAE-Sephacel column chromatography, and the peak of 20β-HSD activity was in good agreement with that of AHR activity. Based on these results, we conclude that 20β-HSD present in liver microsomes of male rats functions as AHR, and exhibits a carbonyl reductase-like activity." @default.
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- W2025681718 title "Further studies on 20β-hydroxysteroid dehydrogenase with carbonyl reductase-like activity present in liver microsomes of male rats" @default.
- W2025681718 doi "https://doi.org/10.1016/s0024-3205(00)00793-1" @default.
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