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- W2025686193 abstract "The retinal pigment epithelial (RPE) cells that underlie the retina ingest and metabolize thousands of lipid-rich photoreceptor outer segments (POS) every day. The scavenger receptor (SR) CD36 and integrin αvβ5 have been shown to participate in POS binding and internalization by RPE cells. The objective of the current study was to determine whether RPE cells express SRs other than CD36. Primary cultures of human RPE cells express both mRNA and protein for SR-BI and SR- BII. SR-BI and SR-BII mRNAs were detected by reverse transcription–polymerase chain reaction. SR-BI protein was detected by immune precipitation of [35S]methionine-labeled crude cellular extracts. SR-BII was detected by Western blotting of immune precipitated crude cellular extracts. SR-BI and SR-BII proteins were also detected by immunofluorescence staining of RPE cells in culture. The results suggest that these SRs may play a role in POS lipid binding and uptake by RPE cells in the eye." @default.
- W2025686193 created "2016-06-24" @default.
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- W2025686193 date "2002-04-01" @default.
- W2025686193 modified "2023-10-17" @default.
- W2025686193 title "Human Retinal Pigment Epithelial Cells Express Scavenger Receptors BI and BII" @default.
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- W2025686193 doi "https://doi.org/10.1006/bbrc.2002.6756" @default.
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