Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025691803> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2025691803 endingPage "2548" @default.
- W2025691803 startingPage "2542" @default.
- W2025691803 abstract "Abstract Affinity‐based reversed micellar extraction and separation (ARMES) has proven effective in separating glycoproteins from nonglycosylated proteins from natural sources. The ability of ARMES to resolve closely related glycoproteins is of paramount importance if ARMES is to be used in glycoform resolution. It is demonstrated that ARMES can resolve the structurally similar soybean peroxidase (SBP; MW 37 kDa, pI 4.1) and α t ‐acid glycoprotein (AGP; MW 43 kDa, pI 3.7), both of which have affinity for Concanavalin A (Con A) (the affinity ligand). SBP was almost exclusively extracted at pH 8 and above, with a separation factor greater than 50 (resolution ∼ 20), far better than was possible using Con A affinity chromatography (R ∼ 0.25, separation factor ∼ 2). Model calculations suggest that differences in affinity measured by an equilibrium‐building assay cannot account for the favorable extraction of SBP over AGP at higher pH. Hydrophobic interactions and/or charge shielding appear to affect partitioning of the lectin ‐ glycoprotein complexes and add greatly to the selectivity of extraction in ARMES, especially at higher pH values." @default.
- W2025691803 created "2016-06-24" @default.
- W2025691803 creator A5025828390 @default.
- W2025691803 creator A5043598718 @default.
- W2025691803 creator A5056210370 @default.
- W2025691803 creator A5090628927 @default.
- W2025691803 date "1998-11-01" @default.
- W2025691803 modified "2023-10-01" @default.
- W2025691803 title "Resolution of glycoproteins by affinity-based reversed micellar extraction and separation" @default.
- W2025691803 cites W1968585653 @default.
- W2025691803 cites W1985286777 @default.
- W2025691803 cites W1998581482 @default.
- W2025691803 cites W2004661062 @default.
- W2025691803 cites W2009505990 @default.
- W2025691803 cites W2020584789 @default.
- W2025691803 cites W2050079367 @default.
- W2025691803 cites W2051622472 @default.
- W2025691803 cites W2062508173 @default.
- W2025691803 cites W2066428971 @default.
- W2025691803 cites W2077433650 @default.
- W2025691803 cites W2082183182 @default.
- W2025691803 cites W2089635636 @default.
- W2025691803 cites W2089930090 @default.
- W2025691803 cites W2117718677 @default.
- W2025691803 cites W284312488 @default.
- W2025691803 doi "https://doi.org/10.1002/aic.690441121" @default.
- W2025691803 hasPublicationYear "1998" @default.
- W2025691803 type Work @default.
- W2025691803 sameAs 2025691803 @default.
- W2025691803 citedByCount "7" @default.
- W2025691803 countsByYear W20256918032013 @default.
- W2025691803 countsByYear W20256918032016 @default.
- W2025691803 crossrefType "journal-article" @default.
- W2025691803 hasAuthorship W2025691803A5025828390 @default.
- W2025691803 hasAuthorship W2025691803A5043598718 @default.
- W2025691803 hasAuthorship W2025691803A5056210370 @default.
- W2025691803 hasAuthorship W2025691803A5090628927 @default.
- W2025691803 hasConcept C108625454 @default.
- W2025691803 hasConcept C116569031 @default.
- W2025691803 hasConcept C118792377 @default.
- W2025691803 hasConcept C138268822 @default.
- W2025691803 hasConcept C154945302 @default.
- W2025691803 hasConcept C161790260 @default.
- W2025691803 hasConcept C170493617 @default.
- W2025691803 hasConcept C179247698 @default.
- W2025691803 hasConcept C181199279 @default.
- W2025691803 hasConcept C185592680 @default.
- W2025691803 hasConcept C19869320 @default.
- W2025691803 hasConcept C202751555 @default.
- W2025691803 hasConcept C2780758401 @default.
- W2025691803 hasConcept C41008148 @default.
- W2025691803 hasConcept C43617362 @default.
- W2025691803 hasConcept C4725764 @default.
- W2025691803 hasConcept C55493867 @default.
- W2025691803 hasConceptScore W2025691803C108625454 @default.
- W2025691803 hasConceptScore W2025691803C116569031 @default.
- W2025691803 hasConceptScore W2025691803C118792377 @default.
- W2025691803 hasConceptScore W2025691803C138268822 @default.
- W2025691803 hasConceptScore W2025691803C154945302 @default.
- W2025691803 hasConceptScore W2025691803C161790260 @default.
- W2025691803 hasConceptScore W2025691803C170493617 @default.
- W2025691803 hasConceptScore W2025691803C179247698 @default.
- W2025691803 hasConceptScore W2025691803C181199279 @default.
- W2025691803 hasConceptScore W2025691803C185592680 @default.
- W2025691803 hasConceptScore W2025691803C19869320 @default.
- W2025691803 hasConceptScore W2025691803C202751555 @default.
- W2025691803 hasConceptScore W2025691803C2780758401 @default.
- W2025691803 hasConceptScore W2025691803C41008148 @default.
- W2025691803 hasConceptScore W2025691803C43617362 @default.
- W2025691803 hasConceptScore W2025691803C4725764 @default.
- W2025691803 hasConceptScore W2025691803C55493867 @default.
- W2025691803 hasIssue "11" @default.
- W2025691803 hasLocation W20256918031 @default.
- W2025691803 hasOpenAccess W2025691803 @default.
- W2025691803 hasPrimaryLocation W20256918031 @default.
- W2025691803 hasRelatedWork W1829823232 @default.
- W2025691803 hasRelatedWork W1965013033 @default.
- W2025691803 hasRelatedWork W1995457951 @default.
- W2025691803 hasRelatedWork W2028299127 @default.
- W2025691803 hasRelatedWork W2037290241 @default.
- W2025691803 hasRelatedWork W2046202946 @default.
- W2025691803 hasRelatedWork W2049478023 @default.
- W2025691803 hasRelatedWork W2050342772 @default.
- W2025691803 hasRelatedWork W2093467526 @default.
- W2025691803 hasRelatedWork W2404017004 @default.
- W2025691803 hasVolume "44" @default.
- W2025691803 isParatext "false" @default.
- W2025691803 isRetracted "false" @default.
- W2025691803 magId "2025691803" @default.
- W2025691803 workType "article" @default.