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- W2025696304 abstract "Double minutes (dm) characteristically exhibit greater numerical heterogeneity among tumor cells than do chromosomes. The biological basis of this heterogeneity was studied in human carcinoma cell line S 18. Pulse labeling of asynchronous cells with [3H]dThd, continuous labeling of synchronized cells with BrdUrd and prematurely condensed chromosome (PCC) studies of G1 and G2 phase S 18 cells indicate that dm-DNA replicates only once during S phase of the cell cycle. No evidence was found for replication of dm-DNA at G1 phase, G2 phase or mitotis. Cells observed at anaphase show imprecise distribution of dm to daughter cells. These studies suggest numerical heterogeneity of dm results from anomalous mitotic segregation rather than anomalous replication of dmDNA." @default.
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- W2025696304 date "1980-12-01" @default.
- W2025696304 modified "2023-09-27" @default.
- W2025696304 title "Double minutes replicate once during S phase of the cell cycle" @default.
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- W2025696304 doi "https://doi.org/10.1016/0014-4827(80)90012-9" @default.
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