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- W2025706827 abstract "Sparse, random labelling of individual cells is a key approach to study brain circuit organisation and development. An array of methods based on genetic engineering now complements older methods such as Golgi staining, facilitating analysis while providing higher information content. Increasingly refined expression strategies based on transcriptional modulators and site-specific recombinases are used to distribute markers or combinations of markers within specific neuronal subsets. Several trends are emerging: first, increasing labelling density with multiplexed markers to allow more cells to be reliably distinguished; second, using labels to report lineage relationships among defined cells in addition to anatomy; third, coupling cell labelling with genetic manipulations that reveal or perturb cell function. These strategies offer new opportunities for characterizing the fine scale architecture of neuronal circuits, and understanding lineage and functional relations among their cellular components in normal or experimental situations." @default.
- W2025706827 created "2016-06-24" @default.
- W2025706827 creator A5014215458 @default.
- W2025706827 creator A5055440525 @default.
- W2025706827 date "2012-02-01" @default.
- W2025706827 modified "2023-09-26" @default.
- W2025706827 title "Sparse and combinatorial neuron labelling" @default.
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- W2025706827 doi "https://doi.org/10.1016/j.conb.2011.09.010" @default.
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