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- W2025731119 abstract "Docetaxel lipid microsphere (DT-LM) is a novel formulation of docetaxel without Tween-80. A sensitive, robust and reproducible ultrafiltration (UF) followed by UPLC-MS/MS method was developed and validated for the quantification of unbound docetaxel in human plasma using paclitaxel as IS. Ultrafiltrate samples were chromatographed on Acquity UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 μm). The mobile phase was a mixture of 10 mM ammonium formate in water containing 0.2% formic acid (A) and acetonitrile containing 0.2% formic acid (B). The volume of plasma utilized was only 450 μL. The calibration curve was linear over the range of 0.2–200 ng/mL, with LLOQ of 0.2 ng/mL. The method was shown to be reliable and reproducible with intra- and inter-day precision and accuracy <±15%, and extraction recovery of 98.1–104.8%. Docetaxel was stable during stability studies, e.g., short term, post-preparation and freeze–thaw cycles. The validated method was utilized to support the pharmacological study of DT-LM in patients with advanced cancer." @default.
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- W2025731119 date "2014-09-01" @default.
- W2025731119 modified "2023-10-16" @default.
- W2025731119 title "Development and validation of an ultrafiltration-UPLC-MS/MS method for rapid quantification of unbound docetaxel in human plasma" @default.
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- W2025731119 doi "https://doi.org/10.1016/j.jchromb.2014.07.007" @default.
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