FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025759932> ?p ?o ?g. }

• W2025759932 endingPage "961" @default.
• W2025759932 startingPage "951" @default.
• W2025759932 abstract "Cyclic AMP-dependent protein kinase (PKA) targets contractile proteins, troponin-I (TnI) and myosin binding protein C (MyBP-C) in the heart and induces a decrease in myofilament Ca2+ sensitivity. Yet, the effect of sarcomere length (SL) change on Ca2+ sensitivity (length-dependent activation: LDA) following PKA-dependent phosphorylation is not clear. To clarify the role of PKA-dependent phosphorylation of TnI and MyBP-C on LDA in the heart, we examined LDA in skinned myocytes from a non-transgenic (NTG) and a transgenic murine model in which the native cardiac isoform (cTnI) was completely replaced by the slow skeletal isoform of TnI (ssTnI-TG) lacking the phosphorylation sites for PKA, while retaining PKA sites on MyBP-C. In NTG myocytes, PKA treatment decreased Ca2+ sensitivity at each SL, but enhanced the impact of SL change on Ca2+ sensitivity. Despite a greater sensitivity to Ca2+ and a reduction in LDA, neither Ca2+ responsiveness nor LDA was affected by PKA treatment in ssTnI-TG myocytes. To determine whether the above observations could be explained by the lateral separation between thick and thin filaments, as suggested by others, we measured interfilament spacing by X-ray diffraction as a function of SL in skinned cardiac trabeculae in the passive state from both NTG and ssTnI-TG models before and following treatment with PKA. Phosphorylation by PKA increased lattice spacing at every SL in NTG trabeculae. However, the relationship between SL and myofilament lattice spacing in ssTnI-TG was markedly shifted downward to an overall decreased myofilament lattice spacing following PKA treatment. We conclude: (1) PKA-dependent phosphorylation enhances length-dependent activation in NTG hearts; (2) replacement of native TnI with ssTnI increases Ca2+ sensitivity of tension but reduces length-dependent activation; (3) MyBP-C phosphorylation by PKA does not alter calcium responsiveness and induces a decrease in myofilament lattice spacing at all sarcomere lengths and (4) length-dependent activation in the heart cannot be entirely explained by alterations in myofilament lattice spacing." @default.
• W2025759932 created "2016-06-24" @default.
• W2025759932 creator A5014102063 @default.
• W2025759932 creator A5024116930 @default.
• W2025759932 creator A5028046659 @default.
• W2025759932 creator A5034848027 @default.
• W2025759932 creator A5056694490 @default.
• W2025759932 creator A5076808976 @default.
• W2025759932 creator A5086108615 @default.
• W2025759932 date "2003-01-24" @default.
• W2025759932 modified "2023-10-10" @default.
• W2025759932 title "Troponin I in the murine myocardium: influence on length-dependent activation and interfilament spacing" @default.
• W2025759932 cites W1490442168 @default.
• W2025759932 cites W1516256265 @default.
• W2025759932 cites W1523578038 @default.
• W2025759932 cites W1819240213 @default.
• W2025759932 cites W1965246031 @default.
• W2025759932 cites W1966368724 @default.
• W2025759932 cites W1970195157 @default.
• W2025759932 cites W1979392703 @default.
• W2025759932 cites W1985345188 @default.
• W2025759932 cites W1987821757 @default.
• W2025759932 cites W1989255509 @default.
• W2025759932 cites W1992491894 @default.
• W2025759932 cites W1996117515 @default.
• W2025759932 cites W1996288114 @default.
• W2025759932 cites W2006502789 @default.
• W2025759932 cites W2006832542 @default.
• W2025759932 cites W2008186189 @default.
• W2025759932 cites W2014534876 @default.
• W2025759932 cites W2017745285 @default.
• W2025759932 cites W2035949471 @default.
• W2025759932 cites W2038379218 @default.
• W2025759932 cites W2039826528 @default.
• W2025759932 cites W2043130547 @default.
• W2025759932 cites W2045141838 @default.
• W2025759932 cites W2049181319 @default.
• W2025759932 cites W2049567264 @default.
• W2025759932 cites W2050853946 @default.
• W2025759932 cites W2055002273 @default.
• W2025759932 cites W2055578380 @default.
• W2025759932 cites W2068739543 @default.
• W2025759932 cites W2072529314 @default.
• W2025759932 cites W2072614512 @default.
• W2025759932 cites W2091519740 @default.
• W2025759932 cites W2097847010 @default.
• W2025759932 cites W2103624248 @default.
• W2025759932 cites W2105274063 @default.
• W2025759932 cites W2120327065 @default.
• W2025759932 cites W2127917887 @default.
• W2025759932 cites W2128706302 @default.
• W2025759932 cites W2139565971 @default.
• W2025759932 cites W2142178785 @default.
• W2025759932 cites W2142754357 @default.
• W2025759932 cites W2144060453 @default.
• W2025759932 cites W2146643518 @default.
• W2025759932 cites W2150832969 @default.
• W2025759932 cites W2157988621 @default.
• W2025759932 cites W2160408886 @default.
• W2025759932 cites W2164108161 @default.
• W2025759932 cites W2169223381 @default.
• W2025759932 cites W2171530060 @default.
• W2025759932 cites W2185806598 @default.
• W2025759932 cites W2228302834 @default.
• W2025759932 cites W2229458813 @default.
• W2025759932 cites W2401366797 @default.
• W2025759932 cites W2419432294 @default.
• W2025759932 cites W2428721686 @default.
• W2025759932 cites W63512097 @default.
• W2025759932 doi "https://doi.org/10.1113/jphysiol.2002.038117" @default.
• W2025759932 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2342721" @default.
• W2025759932 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12562915" @default.
• W2025759932 hasPublicationYear "2003" @default.
• W2025759932 type Work @default.
• W2025759932 sameAs 2025759932 @default.
• W2025759932 citedByCount "126" @default.
• W2025759932 countsByYear W20257599322012 @default.
• W2025759932 countsByYear W20257599322013 @default.
• W2025759932 countsByYear W20257599322014 @default.
• W2025759932 countsByYear W20257599322015 @default.
• W2025759932 countsByYear W20257599322016 @default.
• W2025759932 countsByYear W20257599322017 @default.
• W2025759932 countsByYear W20257599322018 @default.
• W2025759932 countsByYear W20257599322019 @default.
• W2025759932 countsByYear W20257599322020 @default.
• W2025759932 countsByYear W20257599322021 @default.
• W2025759932 countsByYear W20257599322022 @default.
• W2025759932 countsByYear W20257599322023 @default.
• W2025759932 crossrefType "journal-article" @default.
• W2025759932 hasAuthorship W2025759932A5014102063 @default.
• W2025759932 hasAuthorship W2025759932A5024116930 @default.
• W2025759932 hasAuthorship W2025759932A5028046659 @default.
• W2025759932 hasAuthorship W2025759932A5034848027 @default.
• W2025759932 hasAuthorship W2025759932A5056694490 @default.
• W2025759932 hasAuthorship W2025759932A5076808976 @default.
• W2025759932 hasAuthorship W2025759932A5086108615 @default.
• W2025759932 hasBestOaLocation W20257599322 @default.
• W2025759932 hasConcept C115725540 @default.

• next