FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025859080> ?p ?o ?g. }

• W2025859080 endingPage "174" @default.
• W2025859080 startingPage "168" @default.
• W2025859080 abstract "Biotransformation enzymes can, to a certain extent, protect parasitic worms against the toxic effects of anthelmintics and can contribute to drug-resistance development. The objective of our work was (1) to find and identify phase I and II metabolites of the anthelmintic praziquantel (PZQ) formed by the lancet fluke (Dicrocoelium dendriticum) and the rat tapeworm (Hymenolepis diminuta) and (2) to compare PZQ metabolites in helminths with PZQ biotransformation in rat as host species. Ultra high performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS) was used for this purpose. During in vitro incubations, mitochondria-like and microsomes-like fractions (prepared from homogenates of adult worms or from rat liver homogenate) were incubated with 10 and 100 μM PZQ. Liquid/liquid extraction was used for samples during in vitro experiments. In the ex vivo study, living D. dendriticum and H. diminuta adults were incubated in RPMI-1640 medium in the presence of 50 nM or 100 nM PZQ for 24h. After incubation, the worms were removed from the medium and homogenized. Homogenates of worms, medium from the incubation of worms or rat hepatocytes and rat urine (collected during 24h after oral PZQ administration) were separately extracted using solid-phase extraction. The results showed that both D. dendriticum and H. diminuta enzymatic systems are not able to metabolize PZQ. On the other hand, thirty one different phase I and four phase II PZQ metabolites were detected in rat samples using UHPLC/MS/MS analyses. These results show that our experimental helminths, as the members of tapeworm and fluke groups of parasites, are not able to deactivate PZQ, and that the biotransformation enzymes of the studied helminths do not contribute to PZQ-resistance." @default.
• W2025859080 created "2016-06-24" @default.
• W2025859080 creator A5000141639 @default.
• W2025859080 creator A5002827640 @default.
• W2025859080 creator A5004289752 @default.
• W2025859080 creator A5021912922 @default.
• W2025859080 creator A5032817467 @default.
• W2025859080 creator A5034531774 @default.
• W2025859080 creator A5036401008 @default.
• W2025859080 creator A5057012496 @default.
• W2025859080 date "2012-04-01" @default.
• W2025859080 modified "2023-10-18" @default.
• W2025859080 title "The inability of tapeworm Hymenolepis diminuta and fluke Dicrocoelium dendriticum to metabolize praziquantel" @default.
• W2025859080 cites W1968746344 @default.
• W2025859080 cites W1983599170 @default.
• W2025859080 cites W1990770244 @default.
• W2025859080 cites W1995752312 @default.
• W2025859080 cites W2005880092 @default.
• W2025859080 cites W2008824632 @default.
• W2025859080 cites W2021889036 @default.
• W2025859080 cites W2026115685 @default.
• W2025859080 cites W2033230327 @default.
• W2025859080 cites W2041521849 @default.
• W2025859080 cites W2061853758 @default.
• W2025859080 cites W2063936987 @default.
• W2025859080 cites W2067454999 @default.
• W2025859080 cites W2080796517 @default.
• W2025859080 cites W2086626993 @default.
• W2025859080 cites W2124538952 @default.
• W2025859080 cites W2133160678 @default.
• W2025859080 cites W2165567051 @default.
• W2025859080 cites W4234390511 @default.
• W2025859080 doi "https://doi.org/10.1016/j.vetpar.2011.09.026" @default.
• W2025859080 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21996005" @default.
• W2025859080 hasPublicationYear "2012" @default.
• W2025859080 type Work @default.
• W2025859080 sameAs 2025859080 @default.
• W2025859080 citedByCount "12" @default.
• W2025859080 countsByYear W20258590802012 @default.
• W2025859080 countsByYear W20258590802013 @default.
• W2025859080 countsByYear W20258590802014 @default.
• W2025859080 countsByYear W20258590802015 @default.
• W2025859080 countsByYear W20258590802016 @default.
• W2025859080 countsByYear W20258590802017 @default.
• W2025859080 countsByYear W20258590802022 @default.
• W2025859080 crossrefType "journal-article" @default.
• W2025859080 hasAuthorship W2025859080A5000141639 @default.
• W2025859080 hasAuthorship W2025859080A5002827640 @default.
• W2025859080 hasAuthorship W2025859080A5004289752 @default.
• W2025859080 hasAuthorship W2025859080A5021912922 @default.
• W2025859080 hasAuthorship W2025859080A5032817467 @default.
• W2025859080 hasAuthorship W2025859080A5034531774 @default.
• W2025859080 hasAuthorship W2025859080A5036401008 @default.
• W2025859080 hasAuthorship W2025859080A5057012496 @default.
• W2025859080 hasConcept C13590703 @default.
• W2025859080 hasConcept C136764020 @default.
• W2025859080 hasConcept C165901193 @default.
• W2025859080 hasConcept C181199279 @default.
• W2025859080 hasConcept C202751555 @default.
• W2025859080 hasConcept C203014093 @default.
• W2025859080 hasConcept C25642318 @default.
• W2025859080 hasConcept C2776225656 @default.
• W2025859080 hasConcept C2776319035 @default.
• W2025859080 hasConcept C2776908094 @default.
• W2025859080 hasConcept C2779452502 @default.
• W2025859080 hasConcept C2779744950 @default.
• W2025859080 hasConcept C27881333 @default.
• W2025859080 hasConcept C41008148 @default.
• W2025859080 hasConcept C55493867 @default.
• W2025859080 hasConcept C71928629 @default.
• W2025859080 hasConcept C86803240 @default.
• W2025859080 hasConcept C89423630 @default.
• W2025859080 hasConcept C90856448 @default.
• W2025859080 hasConcept C98274493 @default.
• W2025859080 hasConceptScore W2025859080C13590703 @default.
• W2025859080 hasConceptScore W2025859080C136764020 @default.
• W2025859080 hasConceptScore W2025859080C165901193 @default.
• W2025859080 hasConceptScore W2025859080C181199279 @default.
• W2025859080 hasConceptScore W2025859080C202751555 @default.
• W2025859080 hasConceptScore W2025859080C203014093 @default.
• W2025859080 hasConceptScore W2025859080C25642318 @default.
• W2025859080 hasConceptScore W2025859080C2776225656 @default.
• W2025859080 hasConceptScore W2025859080C2776319035 @default.
• W2025859080 hasConceptScore W2025859080C2776908094 @default.
• W2025859080 hasConceptScore W2025859080C2779452502 @default.
• W2025859080 hasConceptScore W2025859080C2779744950 @default.
• W2025859080 hasConceptScore W2025859080C27881333 @default.
• W2025859080 hasConceptScore W2025859080C41008148 @default.
• W2025859080 hasConceptScore W2025859080C55493867 @default.
• W2025859080 hasConceptScore W2025859080C71928629 @default.
• W2025859080 hasConceptScore W2025859080C86803240 @default.
• W2025859080 hasConceptScore W2025859080C89423630 @default.
• W2025859080 hasConceptScore W2025859080C90856448 @default.
• W2025859080 hasConceptScore W2025859080C98274493 @default.
• W2025859080 hasFunder F4320309755 @default.
• W2025859080 hasFunder F4320321006 @default.
• W2025859080 hasIssue "2-4" @default.
• W2025859080 hasLocation W20258590801 @default.

• next