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- W2025862668 abstract "Monoclonal antibodies against cardiac myosin from chicken (18d embryos) and rabbit were produced in vitro by fusion of mouse myeloma cells (P3-X63-Ag8) with spleen cells from immunized BALB/c mice and Lewis rats. Three antibodies were examined and found to react with different antigenic determinants in the myosin heavy chain subunit. Antibody RCM-79 reacted specifically with heavy meromyosin. Antibodies CCM-13 and RCM-79 reacted with equal affinities to cardiac myosin from chicken, rabbit, rat, and man, but with lower affinities to skeletal muscle myosins from chicken and rabbit. Antibody CCM-31A reacted only to chicken cardiac myosin and not to other cardiac or skeletal myosins tested. Our results show that monoclonal antibodies can be produced that are either specific for sequences common to all of the cardiac myosins examined or unique to the immunizing type." @default.
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- W2025862668 date "1980-08-01" @default.
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- W2025862668 title "Characterization of monoclonal antibodies directed against determinants on cardiac myosin heavy chain" @default.
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- W2025862668 doi "https://doi.org/10.1016/s0006-291x(80)80092-1" @default.
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