Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025951200> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2025951200 endingPage "138" @default.
- W2025951200 startingPage "133" @default.
- W2025951200 abstract "The novel high-affinity competitive NMDA receptor antagonist, CGP39653, is employed as radioligand to autoradiographically label the NMDA receptor in rat and human brain. Glutamate dehydrogenase (GlDH; E.C.1.4.1.3) was added to the incubation buffer to degrade residual endogenous L-glutamate, which was not entirely removed from the section after the prewashing step and interfered with [3H]CGP39653 binding. At 20 nM [3H]CGP39653, GLDH increased specific binding as much as 5 times, depending on the dose of GlDH and the presence of NAD+, and hydrazine. Scatchard plots of binding data revealed that this increase was due to a decrease of the KD from 148 nM to 33 nM in the absence and the presence of GlDH, respectively. Addition of GlDH in the NMDA receptor autoradiographic assay may be of importance in quantitative studies with human brain tissue which may contain variable levels of endogenous L-glutamate." @default.
- W2025951200 created "2016-06-24" @default.
- W2025951200 creator A5005208872 @default.
- W2025951200 creator A5022641762 @default.
- W2025951200 creator A5040676309 @default.
- W2025951200 date "1993-02-01" @default.
- W2025951200 modified "2023-09-23" @default.
- W2025951200 title "Glutamate dehydrogenase improves binding of [3H]CGP39653 to NMDA receptors in the autoradiographic assay" @default.
- W2025951200 cites W1964899065 @default.
- W2025951200 cites W1970388657 @default.
- W2025951200 cites W1980378724 @default.
- W2025951200 cites W2002214235 @default.
- W2025951200 cites W2048050987 @default.
- W2025951200 cites W2050553238 @default.
- W2025951200 cites W2061718284 @default.
- W2025951200 cites W2070933799 @default.
- W2025951200 cites W2088645019 @default.
- W2025951200 cites W2100040827 @default.
- W2025951200 cites W2161612105 @default.
- W2025951200 doi "https://doi.org/10.1016/0165-0270(93)90148-k" @default.
- W2025951200 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7682638" @default.
- W2025951200 hasPublicationYear "1993" @default.
- W2025951200 type Work @default.
- W2025951200 sameAs 2025951200 @default.
- W2025951200 citedByCount "19" @default.
- W2025951200 countsByYear W20259512002021 @default.
- W2025951200 crossrefType "journal-article" @default.
- W2025951200 hasAuthorship W2025951200A5005208872 @default.
- W2025951200 hasAuthorship W2025951200A5022641762 @default.
- W2025951200 hasAuthorship W2025951200A5040676309 @default.
- W2025951200 hasConcept C107824862 @default.
- W2025951200 hasConcept C153911025 @default.
- W2025951200 hasConcept C16613235 @default.
- W2025951200 hasConcept C170493617 @default.
- W2025951200 hasConcept C185592680 @default.
- W2025951200 hasConcept C25642318 @default.
- W2025951200 hasConcept C2777202666 @default.
- W2025951200 hasConcept C2780648746 @default.
- W2025951200 hasConcept C55493867 @default.
- W2025951200 hasConcept C61174792 @default.
- W2025951200 hasConcept C67018056 @default.
- W2025951200 hasConcept C67847695 @default.
- W2025951200 hasConcept C86803240 @default.
- W2025951200 hasConceptScore W2025951200C107824862 @default.
- W2025951200 hasConceptScore W2025951200C153911025 @default.
- W2025951200 hasConceptScore W2025951200C16613235 @default.
- W2025951200 hasConceptScore W2025951200C170493617 @default.
- W2025951200 hasConceptScore W2025951200C185592680 @default.
- W2025951200 hasConceptScore W2025951200C25642318 @default.
- W2025951200 hasConceptScore W2025951200C2777202666 @default.
- W2025951200 hasConceptScore W2025951200C2780648746 @default.
- W2025951200 hasConceptScore W2025951200C55493867 @default.
- W2025951200 hasConceptScore W2025951200C61174792 @default.
- W2025951200 hasConceptScore W2025951200C67018056 @default.
- W2025951200 hasConceptScore W2025951200C67847695 @default.
- W2025951200 hasConceptScore W2025951200C86803240 @default.
- W2025951200 hasIssue "2" @default.
- W2025951200 hasLocation W20259512001 @default.
- W2025951200 hasLocation W20259512002 @default.
- W2025951200 hasOpenAccess W2025951200 @default.
- W2025951200 hasPrimaryLocation W20259512001 @default.
- W2025951200 hasRelatedWork W1967659964 @default.
- W2025951200 hasRelatedWork W1976651454 @default.
- W2025951200 hasRelatedWork W1987380889 @default.
- W2025951200 hasRelatedWork W2003337582 @default.
- W2025951200 hasRelatedWork W2025951200 @default.
- W2025951200 hasRelatedWork W2069908052 @default.
- W2025951200 hasRelatedWork W2292662545 @default.
- W2025951200 hasRelatedWork W2467725232 @default.
- W2025951200 hasRelatedWork W2561478473 @default.
- W2025951200 hasRelatedWork W54842643 @default.
- W2025951200 hasVolume "46" @default.
- W2025951200 isParatext "false" @default.
- W2025951200 isRetracted "false" @default.
- W2025951200 magId "2025951200" @default.
- W2025951200 workType "article" @default.