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- W2025951607 abstract "Methods for efficient extraction and simple purification of the lipopolysaccharide specific for Mycobacterium tuberculosis were developed. Crude lipopolysaccharide was obtained from sterilized cells through mechanical disintegration, Triton X-100 extraction, ethanol precipitation, glucosidase digestion, and gel-filtration chromatography. The lipopolysaccharide was further purified by treatments with pyridine-methanol and chloroform-methanol to remove the contaminating glycolipids and phospholipids, and by digestion with the immobilized trypsin to remove the contaminating proteins. The purified lipopolysaccharide was composed of a polysaccharide consisting of d-mannose and d-arabinose, and fatty acids, mainly palmitic, tuberculostearic, and stearic acids, which were bound in ester linkages. The lipopolysaccharide had strong tumor regressing activity on the mouse fibrosarcoma." @default.
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- W2025951607 date "1987-01-01" @default.
- W2025951607 modified "2023-09-25" @default.
- W2025951607 title "Lipopolysaccharide isolated from Mycobacterium tuberculosis strain Aoyama B." @default.
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- W2025951607 doi "https://doi.org/10.1271/bbb1961.51.691" @default.
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