FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025972202> ?p ?o ?g. }

• W2025972202 endingPage "2106" @default.
• W2025972202 startingPage "2088" @default.
• W2025972202 abstract "Despite their potent antimicrobial activity, the usefulness of antimicrobial peptides (AMPs) as antibiotics has been limited by their toxicity to eukaryotic cells and a lack of stability in vivo. In the present study we examined the effects of introducing D-lysine residues into a 15-residue hybrid AMP containing residues 1–7 of cecropin A and residues 2–9 of melittin (designated CM15). Diastereomeric analogs of CM15 containing between two and five D-lysine substitutions were evaluated for their antimicrobial activity, lysis of human erythrocytes, toxicity to murine macrophages, ability to disrupt cell membranes, and protease stability. All of the analogs caused rapid permeabilization of the Staphylococcus aureus cell envelope, as indicated by uptake of SYTOX green. Permeabilization of the plasma membrane of RAW264.7 macrophages was also observed for CM15, but this was substantially diminished for the D-lysine containing analogs. The introduction of D-lysine caused moderate decreases in antimicrobial activity for all analogs studied, with a much more pronounced reduction in toxicity to eukaryotic cells, leading to marked improvements in antimicrobial efficacy. Circular dichroism studies indicated a progressive loss of helical secondary structure upon introduction of D-lysine residues, with a good correspondence between helical content and eukaryotic cell cytotoxicity. Overall, these studies indicate that disruption of amphipathic secondary structure reduces both antimicrobial activity and eukaryotic cell toxicity, but that the reduction in eukaryotic cell cytotoxicity is more pronounced, leading to an overall gain in antimicrobial selectivity." @default.
• W2025972202 created "2016-06-24" @default.
• W2025972202 creator A5024996281 @default.
• W2025972202 creator A5045874970 @default.
• W2025972202 date "2011-12-06" @default.
• W2025972202 modified "2023-09-27" @default.
• W2025972202 title "Effects of D-Lysine Substitutions on the Activity and Selectivity of Antimicrobial Peptide CM15" @default.
• W2025972202 cites W1516030996 @default.
• W2025972202 cites W1964323868 @default.
• W2025972202 cites W1965738364 @default.
• W2025972202 cites W1971040914 @default.
• W2025972202 cites W1971756197 @default.
• W2025972202 cites W1975954217 @default.
• W2025972202 cites W1978029175 @default.
• W2025972202 cites W1984679573 @default.
• W2025972202 cites W1986413623 @default.
• W2025972202 cites W1989998611 @default.
• W2025972202 cites W1993842357 @default.
• W2025972202 cites W1994567110 @default.
• W2025972202 cites W1995278782 @default.
• W2025972202 cites W1997152791 @default.
• W2025972202 cites W2000687163 @default.
• W2025972202 cites W2005929034 @default.
• W2025972202 cites W2007331931 @default.
• W2025972202 cites W2007408986 @default.
• W2025972202 cites W2009106357 @default.
• W2025972202 cites W2011832224 @default.
• W2025972202 cites W2014550271 @default.
• W2025972202 cites W2018984543 @default.
• W2025972202 cites W2019834300 @default.
• W2025972202 cites W2024313783 @default.
• W2025972202 cites W2031966010 @default.
• W2025972202 cites W2033577193 @default.
• W2025972202 cites W2038512626 @default.
• W2025972202 cites W2040172698 @default.
• W2025972202 cites W2041189201 @default.
• W2025972202 cites W2046501778 @default.
• W2025972202 cites W2054602599 @default.
• W2025972202 cites W2056013343 @default.
• W2025972202 cites W2058952776 @default.
• W2025972202 cites W2071718874 @default.
• W2025972202 cites W2082948099 @default.
• W2025972202 cites W2084246486 @default.
• W2025972202 cites W2084699782 @default.
• W2025972202 cites W2084878620 @default.
• W2025972202 cites W2087125825 @default.
• W2025972202 cites W2088751086 @default.
• W2025972202 cites W2089106886 @default.
• W2025972202 cites W2092699435 @default.
• W2025972202 cites W2097108780 @default.
• W2025972202 cites W2105379169 @default.
• W2025972202 cites W2111584103 @default.
• W2025972202 cites W2115622227 @default.
• W2025972202 cites W2118486559 @default.
• W2025972202 cites W2126224226 @default.
• W2025972202 cites W2127419087 @default.
• W2025972202 cites W2127616123 @default.
• W2025972202 cites W2133056039 @default.
• W2025972202 cites W2136906409 @default.
• W2025972202 cites W2141356097 @default.
• W2025972202 cites W2148371058 @default.
• W2025972202 cites W2159861471 @default.
• W2025972202 cites W2160780417 @default.
• W2025972202 cites W2161964027 @default.
• W2025972202 cites W2163251457 @default.
• W2025972202 cites W2168482920 @default.
• W2025972202 cites W2169459642 @default.
• W2025972202 cites W2170912840 @default.
• W2025972202 cites W23223599 @default.
• W2025972202 cites W4232612684 @default.
• W2025972202 cites W4255507877 @default.
• W2025972202 doi "https://doi.org/10.3390/polym3042088" @default.
• W2025972202 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6217857" @default.
• W2025972202 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30405905" @default.
• W2025972202 hasPublicationYear "2011" @default.
• W2025972202 type Work @default.
• W2025972202 sameAs 2025972202 @default.
• W2025972202 citedByCount "22" @default.
• W2025972202 countsByYear W20259722022013 @default.
• W2025972202 countsByYear W20259722022016 @default.
• W2025972202 countsByYear W20259722022017 @default.
• W2025972202 countsByYear W20259722022018 @default.
• W2025972202 countsByYear W20259722022019 @default.
• W2025972202 countsByYear W20259722022020 @default.
• W2025972202 countsByYear W20259722022021 @default.
• W2025972202 countsByYear W20259722022022 @default.
• W2025972202 countsByYear W20259722022023 @default.
• W2025972202 crossrefType "journal-article" @default.
• W2025972202 hasAuthorship W2025972202A5024996281 @default.
• W2025972202 hasAuthorship W2025972202A5045874970 @default.
• W2025972202 hasBestOaLocation W20259722021 @default.
• W2025972202 hasConcept C109316439 @default.
• W2025972202 hasConcept C185592680 @default.
• W2025972202 hasConcept C202751555 @default.
• W2025972202 hasConcept C2776016237 @default.
• W2025972202 hasConcept C2779281246 @default.
• W2025972202 hasConcept C4937899 @default.
• W2025972202 hasConcept C515207424 @default.

• next