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- W2026013887 abstract "We describe a glucose sensor based on a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry. The GGBP fromEscherichia coliwas mutated to contain a single cysteine residue at position 26. When labeled with a sulfhydryl-reactive probe 2-(4′-iodoacetamidoanilino)naphthalene-6-sulfonic acid, the labeled protein displayed a twofold decrease in intensity in response to glucose, with a dissociation constant near 1 μM glucose. The ANS-labeled protein displayed only a modest change in lifetime, precluding lifetime-based sensing of glucose. A modulation sensor was created by combining ANS26-GGBP with a long-lifetime ruthenium (Ru) metal–ligand complex on the surface of the cuvette. Binding of glucose changed the relative intensity of ANS26-GGBP and the Ru complex, resulting in a dramatic change in modulation at a low frequency of 2.1 MHz. Modulation measurements at 2.1 MHz were shown to accurately determine the glucose concentration. These results suggest an approach to glucose sensing with simple devices." @default.
- W2026013887 created "2016-06-24" @default.
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- W2026013887 date "1999-02-01" @default.
- W2026013887 modified "2023-10-15" @default.
- W2026013887 title "Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein" @default.
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- W2026013887 doi "https://doi.org/10.1006/abio.1998.2974" @default.
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