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- W2026044597 abstract "Abstract A Thermomonospora curvata endoglucanase gene was subcloned into Escherichia coli-Bacillus subtilis shuttle vector pLP1202. E. coli strain DH1 harboring the recombinant cellulase plasmid pTC15 produced an endoglucanase with pH and temperature optima of 8.0–8.5 and 70°C, respectively. This cellulase was produced during exponential growth with >80% secretion (∼60% in periplasmic space and ∼25% in culture fluid). The activity of the enzyme was inhibited by antiserum produced against crude T. curvata extracellular proteins. Expression of endoglucanase was not detected in B. subtilis transformants harboring plasmids containing a functional T. curvata endoglucanase gene." @default.
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- W2026044597 date "1993-06-01" @default.
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- W2026044597 title "Characterization of a Thermomonospora curvata endoglucanase expressed in Escherichia coli" @default.
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