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- W2026068373 abstract "Aggregation of peritoneal exudate cells (PEC) from Bacillus Calmette-Guerin-immunized guinea pigs in the presence of purified protein derivative was completely inhibited when actinomycin D or puromycin was added at the beginning of incubation. The addition of actinomycin D 5 hr after incubation of sensitive PEC with specific antigen no longer inhibited aggregation. When puromycin was added 1 hr before aggregation occurred, the reaction was inhibited. Moreover, the addition of puromycin to cells which had aggregated caused the disappearance of aggregates within 24 hr. The production of macrophage aggregation factor (MAF) by sensitive lymphoid cells appears to require DNA-dependent RNA and protein synthesis. In addition, aggregation of nonsensitive PEC in the presence of active MAF was completely inhibited when actinomycin D was added in the first hour or puromycin was added during the second hour of incubation. These observations suggest that protein metabolism of macrophages must remain intact in order for aggregation to occur in the presence of MAF." @default.
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- W2026068373 date "1971-10-01" @default.
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- W2026068373 title "Inhibition of macrophage aggregation in vitro by actinomycin D and puromycin" @default.
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- W2026068373 doi "https://doi.org/10.1016/0008-8749(71)90050-5" @default.
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