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- W2026085144 abstract "On-line coupled immunoaffinity chromatography–reversed-phase high-performance liquid chromatography (IAC–HPLC) with detection by quadrupole ion trap mass spectrometry using a particle beam interface has been developed for the determination of the steroids, dexamethasone and flumethasone. HEMA (polyhydroxyethylmethacrylate) was evaluated as a support material for the anti-dexamethasone antibodies used in IAC. Antibody cross-reactivity and non-specific binding have been investigated for the HEMA bound anti-dexamethasone IAC column. The on-line IAC–HPLC–MS determination of dexamethasone and flumethasone in post-administration equine urine samples showed precisions (R.S.D.) of 8.0 and 7.1%, respectively, with limits of detection in the range 3–4 ng/ml." @default.
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- W2026085144 date "1998-01-01" @default.
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- W2026085144 title "Immunoaffinity chromatography combined on-line with high-performance liquid chromatography–mass spectrometry for the determination of corticosteroids" @default.
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- W2026085144 doi "https://doi.org/10.1016/s0021-9673(97)00888-1" @default.
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