FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026163322> ?p ?o ?g. }

• W2026163322 endingPage "12973" @default.
• W2026163322 startingPage "12967" @default.
• W2026163322 abstract "Human polynucleotide kinase (hPNK) is a putative DNA repair enzyme in the base excision repair pathway required for processing and rejoining strand-break termini. This study represents the first systematic examination of the physical properties of this enzyme. The protein was produced in Escherichia coli as a His-tagged protein, and the purified recombinant protein exhibited both the kinase and the phosphatase activities. The predicted relative molecular mass (Mr) of the 521 amino acid polypeptide encoded by the sequenced cDNA for PNK and the additional 21 amino acids of the His tag is 59 538. The Mr determined by low-speed sedimentation equilibrium under nondenaturing conditions was 59 600 ± 1000, indicating that the protein exists as a monomer, in contrast to T4 phage PNK, which exists as a homotetramer. The size and shape of hPNK in solution were determined by analytical ultracentrifugation studies. The protein was found to have an intrinsic sedimentation coefficient, s020,w, of 3.54 S and a Stokes radius, Rs, of 37.5 Å. These hydrodynamic data, together with the Mr of 59 600, suggest that hPNK is a moderately asymmetric protein with an axial ratio of 5.51. Analysis of the secondary structure of hPNK on the basis of circular dichroism spectra, which revealed the presence of two negative dichroic bands located at 218 and 209 nm, with ellipticity values of −7200 ± 300 and −7800 ± 300 deg·cm2·dmol-1, respectively, indicated the presence of approximately 50% β-structure and 25% α-helix. Binding of ATP to the protein induced an increase in β-structure and perturbed tryptophan, tyrosine, and phenylalanine signals observed by aromatic CD and UV difference spectroscopy." @default.
• W2026163322 created "2016-06-24" @default.
• W2026163322 creator A5030147093 @default.
• W2026163322 creator A5049077667 @default.
• W2026163322 creator A5065812167 @default.
• W2026163322 creator A5083022125 @default.
• W2026163322 date "2001-10-01" @default.
• W2026163322 modified "2023-09-27" @default.
• W2026163322 title "Physical Properties of Human Polynucleotide Kinase: Hydrodynamic and Spectroscopic Studies" @default.
• W2026163322 cites W138894669 @default.
• W2026163322 cites W1565638729 @default.
• W2026163322 cites W1607161437 @default.
• W2026163322 cites W1976101795 @default.
• W2026163322 cites W1985374711 @default.
• W2026163322 cites W2023842004 @default.
• W2026163322 cites W2041586774 @default.
• W2026163322 cites W2050087823 @default.
• W2026163322 cites W2056348403 @default.
• W2026163322 cites W2064419458 @default.
• W2026163322 cites W2086670387 @default.
• W2026163322 cites W2090190956 @default.
• W2026163322 cites W2091216577 @default.
• W2026163322 cites W2093950640 @default.
• W2026163322 cites W2094077114 @default.
• W2026163322 cites W2095159899 @default.
• W2026163322 cites W2127698844 @default.
• W2026163322 cites W2170532016 @default.
• W2026163322 cites W2291787412 @default.
• W2026163322 doi "https://doi.org/10.1021/bi011383w" @default.
• W2026163322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11669634" @default.
• W2026163322 hasPublicationYear "2001" @default.
• W2026163322 type Work @default.
• W2026163322 sameAs 2026163322 @default.
• W2026163322 citedByCount "23" @default.
• W2026163322 countsByYear W20261633222018 @default.
• W2026163322 countsByYear W20261633222019 @default.
• W2026163322 countsByYear W20261633222021 @default.
• W2026163322 countsByYear W20261633222022 @default.
• W2026163322 countsByYear W20261633222023 @default.
• W2026163322 crossrefType "journal-article" @default.
• W2026163322 hasAuthorship W2026163322A5030147093 @default.
• W2026163322 hasAuthorship W2026163322A5049077667 @default.
• W2026163322 hasAuthorship W2026163322A5065812167 @default.
• W2026163322 hasAuthorship W2026163322A5083022125 @default.
• W2026163322 hasConcept C104292427 @default.
• W2026163322 hasConcept C104317684 @default.
• W2026163322 hasConcept C111921641 @default.
• W2026163322 hasConcept C133571119 @default.
• W2026163322 hasConcept C136543978 @default.
• W2026163322 hasConcept C1388644 @default.
• W2026163322 hasConcept C153911025 @default.
• W2026163322 hasConcept C166940927 @default.
• W2026163322 hasConcept C178790620 @default.
• W2026163322 hasConcept C181199279 @default.
• W2026163322 hasConcept C185592680 @default.
• W2026163322 hasConcept C187882448 @default.
• W2026163322 hasConcept C2778481663 @default.
• W2026163322 hasConcept C46749446 @default.
• W2026163322 hasConcept C47701112 @default.
• W2026163322 hasConcept C521977710 @default.
• W2026163322 hasConcept C54689828 @default.
• W2026163322 hasConcept C55493867 @default.
• W2026163322 hasConcept C62614982 @default.
• W2026163322 hasConcept C8010536 @default.
• W2026163322 hasConcept C86803240 @default.
• W2026163322 hasConceptScore W2026163322C104292427 @default.
• W2026163322 hasConceptScore W2026163322C104317684 @default.
• W2026163322 hasConceptScore W2026163322C111921641 @default.
• W2026163322 hasConceptScore W2026163322C133571119 @default.
• W2026163322 hasConceptScore W2026163322C136543978 @default.
• W2026163322 hasConceptScore W2026163322C1388644 @default.
• W2026163322 hasConceptScore W2026163322C153911025 @default.
• W2026163322 hasConceptScore W2026163322C166940927 @default.
• W2026163322 hasConceptScore W2026163322C178790620 @default.
• W2026163322 hasConceptScore W2026163322C181199279 @default.
• W2026163322 hasConceptScore W2026163322C185592680 @default.
• W2026163322 hasConceptScore W2026163322C187882448 @default.
• W2026163322 hasConceptScore W2026163322C2778481663 @default.
• W2026163322 hasConceptScore W2026163322C46749446 @default.
• W2026163322 hasConceptScore W2026163322C47701112 @default.
• W2026163322 hasConceptScore W2026163322C521977710 @default.
• W2026163322 hasConceptScore W2026163322C54689828 @default.
• W2026163322 hasConceptScore W2026163322C55493867 @default.
• W2026163322 hasConceptScore W2026163322C62614982 @default.
• W2026163322 hasConceptScore W2026163322C8010536 @default.
• W2026163322 hasConceptScore W2026163322C86803240 @default.
• W2026163322 hasIssue "43" @default.
• W2026163322 hasLocation W20261633221 @default.
• W2026163322 hasLocation W20261633222 @default.
• W2026163322 hasOpenAccess W2026163322 @default.
• W2026163322 hasPrimaryLocation W20261633221 @default.
• W2026163322 hasRelatedWork W1974360852 @default.
• W2026163322 hasRelatedWork W1990227323 @default.
• W2026163322 hasRelatedWork W2017718159 @default.
• W2026163322 hasRelatedWork W2026163322 @default.
• W2026163322 hasRelatedWork W2046491379 @default.
• W2026163322 hasRelatedWork W2065857113 @default.
• W2026163322 hasRelatedWork W2080831534 @default.

• next