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- W2026165164 abstract "Both static and kinetic investigations have been performed on the interaction of calf thymus DNA with Acridine Orange (AO). The binding isotherm obtained spectrophotometrically was analyzed by a site exclusion theory and revealed the presence of two binding modes of dye molecule corresponding to intercalation and dimerization on DNA. In the kinetic experiments focusing on the intercalation mode by the temperature-jump technique, three relaxations were observed. From the analyses of both the relaxation time and amplitude, the two fast relaxations were ascribed to parallel binding reactions including a bimolecular pathway for direct transfer of dye molecule on the polymer and the slow relaxation was attributable to a conformational change of DNA molecules. The binding parameters kinetically obtained suggest that 80% of bound AO is in intercalated form and the remainder is in partially intercalated one as an external bound form." @default.
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- W2026165164 date "1988-03-01" @default.
- W2026165164 modified "2023-10-13" @default.
- W2026165164 title "Kinetics of the Interaction between DNA and Acridine Orange" @default.
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- W2026165164 doi "https://doi.org/10.1246/bcsj.61.643" @default.
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