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- W2026171085 abstract "A fast and sensitive chemiluminescent enzyme-linked immunosorbent assay method to measure pathogenic bacteria, Escherichia coli O157:H7, on immuno-chromatographic membrane was studied. Non-specific binding of proteins on membrane strip was controlled to attain the best performance of immunosensor by optimising the composition of a running buffer. The specificity of the proposed immunostrip was confirmed by conducting experiments for four different micro-organisms. A chemiluminescent signal could be successfully generated from a proposed immunostrip sensing system, and a significant change in the chemiluminescent light intensity with the concentration of target microbes was obtained. E. coli O157:H7 could be quantitatively measured in the range of 1.1 × 103 –1.1 × 107 CFU (colony forming units) mL−1 within 16 min by using the developed chemiluminescent immunostrip." @default.
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- W2026171085 date "2012-05-15" @default.
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- W2026171085 title "Chemiluminescent enzyme-linked immunosorbent assay on a strip to detectEscherichia coliO157:H7" @default.
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- W2026171085 doi "https://doi.org/10.1080/03067311003782682" @default.
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