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- W2026172021 abstract "Abstract A mechanistic study of liquid chromatography under limiting conditions of adsorption (LC LCA) is presented. Polystyrene was evaluated in a THF/n-hexane binary eluent using wide- and narrow-pore silica gels and styrene divinylbenzene-based sorbents. The LCA condition was achieved though a balance between the free energies of exclusion and adsorption. Polymer solubility does not influence the retention volume, with LC LCA occurring in the soluble domain of the cloud point diagram. The molecular weight independent retention (LCA) was not found to significantly vary with the mobile phase flow rate, volume and concentration injected. However, the LCA was strongly influenced by the stationary phase surface chemistry and pore size, as well as the temperature. The limiting conditions of adsorption are, therefore, relatively robust for a given poly-mer-eluent-sorbent combination and depend on the operating and physical characteristics of the chromatograph- Examples of LCLCA conditions are reported herein." @default.
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- W2026172021 date "1999-04-01" @default.
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- W2026172021 title "Mechanism of Liquid Chromatography of Macromolecules under Limiting Conditions of Adsorption" @default.
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- W2026172021 doi "https://doi.org/10.1080/10236669908014180" @default.
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