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• W2026175602 abstract "A subclone, designated CEM-ON, derived from an azaguanine-resistant human leukemic T cell line, CEM-AGR, constitutively secretes a colony-stimulating factor(CSF) which stimulates the production of macrophages from murine bone marrow progenitor cells. This CSF has been purified from serum-free conditioned medium. Highly purified CEM-ON CSF with a specific activity of 4.7X107 units/mg protein was obtained. Amino-terminal sequence analysis showed that the first 27 amino acids were identical to the amino-terminal sequence of the M-CSF (CSF-1) based on the cDNAs for human M-CSF. On SDS-PAGE analysis, CEM-ON CSF had an apparent molecular weight of 33,000–43,000; following reduction with 2-mercaptoethanol, this migrated as a 20,000–24,000 subunit, suggesting a homodimer structure. These results show that a human T cell line, CEM-ON, secretes M-CSF into its medium." @default.
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• W2026175602 date "1988-05-01" @default.
• W2026175602 modified "2023-10-14" @default.
• W2026175602 title "Macrophage colony-stimulating factor is produced by human T lymphoblastoid cell line, CEM-ON: Identification by amino-terminal amino acid sequence analysis" @default.
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• W2026175602 doi "https://doi.org/10.1016/s0006-291x(88)80441-8" @default.
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