FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026183154> ?p ?o ?g. }

• W2026183154 abstract "The hormone 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] promotes differentiation of a number of cell types including HL-60 promyelocytic leukemia cells. It is now established that protein kinase Cbeta (PKCbeta) plays a critical role in HL-60 cell maturation to a monocyte/macrophage phenotype. In the present study, we investigated the importance of PKCbeta levels and activation in 1,25-(OH)2D3-mediated differentiation of HL-60 cells. Cell differentiation promoted by 1,25-(OH)2D3 at 48 hr was 39 +/- 3% (mean +/- SEM) nitroblue tetrazolium (NBT) positive and at 72 hr it was 35 +/- 2% NBT positive and 70% CD14 positive. Thus, promotion of cell differentiation by 20 nM 1,25-(OH)2D3 treatment was maximal at 48-72 hr. When PKCbeta levels and cell differentiation were assayed at 72 hr, treatment with 20 nM 1,25-(OH)2D3 for the initial 6 hr increased PKCbeta levels by 175% but had little effect on cell differentiation (7 +/- 2% NBT positive; 11% CD14 positive). The effect of ionomycin, a calcium ionophore, on PKCbeta levels and cell differentiation also was examined. Alone, 5 microM ionomycin promoted few cells (3% CD14 positive) to differentiate. In contrast, cells treated with 5 microM ionomycin for 66 hr after a 6-hr pretreatment with 20 nM 1,25-(OH)2D3 resulted in 34 +/- 5% NBT positive cells and 73% CD14 positive cells. Quantitatively, this induction of differentiation was identical to that observed in cultures continuously treated with 1,25-(OH)2D3 (35 +/- 2% NBT positive; 70% CD14 positive). Therefore, ionomycin seemed to replace the requirement for the continuous presence of 1,25-(OH)2D3. Chelerythrine chloride (3 microM), a specific PKC inhibitor, blocked differentiation promoted by 1,25-(OH)2D3 alone (82 +/- 2% inhibition) or in sequence with ionomycin (86 +/- 3% inhibition). Taken together, our data show that the capacity of 1,25-(OH)2D3 to both increase PKCbeta levels and activate PKC is utilized to promote HL-60 cell differentiation. These data further suggest that 1,25-(OH)2D3 has a genomic action to increase PKCbeta levels and also a nongenomic action requiring its continuous presence to promote HL-60 cell differentiation." @default.
• W2026183154 created "2016-06-24" @default.
• W2026183154 creator A5024602864 @default.
• W2026183154 creator A5055765396 @default.
• W2026183154 creator A5080245956 @default.
• W2026183154 creator A5085517629 @default.
• W2026183154 creator A5091912561 @default.
• W2026183154 date "1997-10-01" @default.
• W2026183154 modified "2023-09-26" @default.
• W2026183154 title "Promotion of HL-60 cell differentiation by 1,25'dihydroxyvitamin D3 regulation of protein kinase C levels and activity" @default.
• W2026183154 cites W1510828048 @default.
• W2026183154 cites W1514281522 @default.
• W2026183154 cites W1520207758 @default.
• W2026183154 cites W1526569781 @default.
• W2026183154 cites W1531695673 @default.
• W2026183154 cites W1537090264 @default.
• W2026183154 cites W1568862210 @default.
• W2026183154 cites W1571280577 @default.
• W2026183154 cites W1579204819 @default.
• W2026183154 cites W1598674360 @default.
• W2026183154 cites W1635474458 @default.
• W2026183154 cites W1854781184 @default.
• W2026183154 cites W1973613269 @default.
• W2026183154 cites W1974955088 @default.
• W2026183154 cites W1977185700 @default.
• W2026183154 cites W1978010050 @default.
• W2026183154 cites W2012571603 @default.
• W2026183154 cites W2026766570 @default.
• W2026183154 cites W2030627408 @default.
• W2026183154 cites W2037448041 @default.
• W2026183154 cites W2054362917 @default.
• W2026183154 cites W2062252312 @default.
• W2026183154 cites W2080751435 @default.
• W2026183154 cites W2090464853 @default.
• W2026183154 cites W2111550283 @default.
• W2026183154 cites W4293247451 @default.
• W2026183154 doi "https://doi.org/10.1016/s0006-2952(97)00286-4" @default.
• W2026183154 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9354591" @default.
• W2026183154 hasPublicationYear "1997" @default.
• W2026183154 type Work @default.
• W2026183154 sameAs 2026183154 @default.
• W2026183154 citedByCount "35" @default.
• W2026183154 countsByYear W20261831542012 @default.
• W2026183154 countsByYear W20261831542013 @default.
• W2026183154 countsByYear W20261831542015 @default.
• W2026183154 countsByYear W20261831542018 @default.
• W2026183154 crossrefType "journal-article" @default.
• W2026183154 hasAuthorship W2026183154A5024602864 @default.
• W2026183154 hasAuthorship W2026183154A5055765396 @default.
• W2026183154 hasAuthorship W2026183154A5080245956 @default.
• W2026183154 hasAuthorship W2026183154A5085517629 @default.
• W2026183154 hasAuthorship W2026183154A5091912561 @default.
• W2026183154 hasConcept C104317684 @default.
• W2026183154 hasConcept C126322002 @default.
• W2026183154 hasConcept C134018914 @default.
• W2026183154 hasConcept C148738053 @default.
• W2026183154 hasConcept C153911025 @default.
• W2026183154 hasConcept C184235292 @default.
• W2026183154 hasConcept C185592680 @default.
• W2026183154 hasConcept C195794163 @default.
• W2026183154 hasConcept C2776989580 @default.
• W2026183154 hasConcept C2781184567 @default.
• W2026183154 hasConcept C519063684 @default.
• W2026183154 hasConcept C55493867 @default.
• W2026183154 hasConcept C71924100 @default.
• W2026183154 hasConcept C86803240 @default.
• W2026183154 hasConcept C95444343 @default.
• W2026183154 hasConceptScore W2026183154C104317684 @default.
• W2026183154 hasConceptScore W2026183154C126322002 @default.
• W2026183154 hasConceptScore W2026183154C134018914 @default.
• W2026183154 hasConceptScore W2026183154C148738053 @default.
• W2026183154 hasConceptScore W2026183154C153911025 @default.
• W2026183154 hasConceptScore W2026183154C184235292 @default.
• W2026183154 hasConceptScore W2026183154C185592680 @default.
• W2026183154 hasConceptScore W2026183154C195794163 @default.
• W2026183154 hasConceptScore W2026183154C2776989580 @default.
• W2026183154 hasConceptScore W2026183154C2781184567 @default.
• W2026183154 hasConceptScore W2026183154C519063684 @default.
• W2026183154 hasConceptScore W2026183154C55493867 @default.
• W2026183154 hasConceptScore W2026183154C71924100 @default.
• W2026183154 hasConceptScore W2026183154C86803240 @default.
• W2026183154 hasConceptScore W2026183154C95444343 @default.
• W2026183154 hasLocation W20261831541 @default.
• W2026183154 hasLocation W20261831542 @default.
• W2026183154 hasOpenAccess W2026183154 @default.
• W2026183154 hasPrimaryLocation W20261831541 @default.
• W2026183154 hasRelatedWork W1995031879 @default.
• W2026183154 hasRelatedWork W2022651928 @default.
• W2026183154 hasRelatedWork W2041427716 @default.
• W2026183154 hasRelatedWork W2055429740 @default.
• W2026183154 hasRelatedWork W2083666507 @default.
• W2026183154 hasRelatedWork W2122860143 @default.
• W2026183154 hasRelatedWork W2123057090 @default.
• W2026183154 hasRelatedWork W2125379501 @default.
• W2026183154 hasRelatedWork W2131413156 @default.
• W2026183154 hasRelatedWork W2152932292 @default.
• W2026183154 isParatext "false" @default.
• W2026183154 isRetracted "false" @default.
• W2026183154 magId "2026183154" @default.
• W2026183154 workType "article" @default.