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- W2026187307 abstract "DNA replication is normally tightly regulated to ensure the production of only one copy of the genome per cell cycle. However, DNA puffs of the salivary gland giant polytene chromosomes of Sciara coprophila undergo DNA amplification during the normal course of development, overriding this control. This developmental strategy provides more template for the production of large amounts of protein needed for pupation. We have focused on DNA puff II/9A, which amplifies ∼ 17-fold over the rest of the genome. Evidence presented here suggests that DNA amplification at this locus is controlled by the steroid hormone ecdysone, the master regulator of insect development. Explanted, pre-amplification stage salivary glands undergo premature amplification when incubated with ecdysone. Injection of ecdysone into pre-amplification stage larvae induces amplification. Ecdysone also induces transcription of the II/9A genes. We report the presence of a putative ecdysone response element directly adjacent to the origin recognition complex (ORC)-binding site in the II/9A origin and demonstrate that it is efficiently bound by the Sciara ecdysone receptor. These results implicate ecdysone in the regulation of DNA amplification in Sciara and suggest the ecdysone receptor may be the elusive amplification factor. This would be a new role for this transcription factor." @default.
- W2026187307 created "2016-06-24" @default.
- W2026187307 creator A5008805548 @default.
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- W2026187307 creator A5077896863 @default.
- W2026187307 date "2006-11-01" @default.
- W2026187307 modified "2023-09-27" @default.
- W2026187307 title "Ecdysone induces transcription and amplification in Sciara coprophila DNA puff II/9A" @default.
- W2026187307 cites W101761358 @default.
- W2026187307 cites W1507212424 @default.
- W2026187307 cites W1597247843 @default.
- W2026187307 cites W1603587119 @default.
- W2026187307 cites W1956207752 @default.
- W2026187307 cites W1963866783 @default.
- W2026187307 cites W1964242121 @default.
- W2026187307 cites W1970212907 @default.
- W2026187307 cites W1972815947 @default.
- W2026187307 cites W1973885639 @default.
- W2026187307 cites W1974306997 @default.
- W2026187307 cites W1976851982 @default.
- W2026187307 cites W1981939829 @default.
- W2026187307 cites W1982121118 @default.
- W2026187307 cites W1982601116 @default.
- W2026187307 cites W1987465149 @default.
- W2026187307 cites W1989071660 @default.
- W2026187307 cites W1989734587 @default.
- W2026187307 cites W1993521976 @default.
- W2026187307 cites W1994122315 @default.
- W2026187307 cites W1995869445 @default.
- W2026187307 cites W2000517171 @default.
- W2026187307 cites W2000518052 @default.
- W2026187307 cites W2002028814 @default.
- W2026187307 cites W2003527666 @default.
- W2026187307 cites W2006775263 @default.
- W2026187307 cites W2009259676 @default.
- W2026187307 cites W2013051413 @default.
- W2026187307 cites W2016730049 @default.
- W2026187307 cites W2017658415 @default.
- W2026187307 cites W2019920692 @default.
- W2026187307 cites W2022298465 @default.
- W2026187307 cites W2027059638 @default.
- W2026187307 cites W2027696604 @default.
- W2026187307 cites W2028953367 @default.
- W2026187307 cites W2030765769 @default.
- W2026187307 cites W2032279306 @default.
- W2026187307 cites W2036637958 @default.
- W2026187307 cites W2042271211 @default.
- W2026187307 cites W2045327902 @default.
- W2026187307 cites W2045512028 @default.
- W2026187307 cites W2046299859 @default.
- W2026187307 cites W2046763813 @default.
- W2026187307 cites W2046854969 @default.
- W2026187307 cites W2047232878 @default.
- W2026187307 cites W2050493067 @default.
- W2026187307 cites W2052428952 @default.
- W2026187307 cites W2052636744 @default.
- W2026187307 cites W2054172214 @default.
- W2026187307 cites W2055791115 @default.
- W2026187307 cites W2061449183 @default.
- W2026187307 cites W2064623121 @default.
- W2026187307 cites W2066195353 @default.
- W2026187307 cites W2071548413 @default.
- W2026187307 cites W2075557949 @default.
- W2026187307 cites W2078682316 @default.
- W2026187307 cites W2088173957 @default.
- W2026187307 cites W2088241335 @default.
- W2026187307 cites W2088493129 @default.
- W2026187307 cites W2091041905 @default.
- W2026187307 cites W2092515531 @default.
- W2026187307 cites W2096327374 @default.
- W2026187307 cites W2097955339 @default.
- W2026187307 cites W2097978662 @default.
- W2026187307 cites W2098033538 @default.
- W2026187307 cites W2099251866 @default.
- W2026187307 cites W2101448549 @default.
- W2026187307 cites W2107405297 @default.
- W2026187307 cites W2108450183 @default.
- W2026187307 cites W2113194068 @default.
- W2026187307 cites W2116537012 @default.
- W2026187307 cites W2117301813 @default.
- W2026187307 cites W2120036606 @default.
- W2026187307 cites W2121524269 @default.
- W2026187307 cites W2128168784 @default.
- W2026187307 cites W2128960755 @default.
- W2026187307 cites W2129044144 @default.
- W2026187307 cites W2131968390 @default.
- W2026187307 cites W2136267625 @default.
- W2026187307 cites W2139305659 @default.
- W2026187307 cites W2142611898 @default.
- W2026187307 cites W2145535073 @default.
- W2026187307 cites W2146921384 @default.
- W2026187307 cites W2157708131 @default.
- W2026187307 cites W2159539440 @default.