Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026240692> ?p ?o ?g. }
- W2026240692 endingPage "52" @default.
- W2026240692 startingPage "46" @default.
- W2026240692 abstract "Nanodiscs are small-sized and flat model membranes that provide a close to native environment for reconstitution of integral membrane proteins. Incorporation of membrane proteins into nanodiscs results in water-soluble proteolipid particles making the membrane proteins amenable to a multitude of bioanalytical techniques originally developed for soluble proteins. The transmembrane domain of the human CD4 receptor was fused to ubiquitin with a preceding N-terminal decahistidine tag. The resulting integral membrane protein was incorporated into nanodiscs. Binding of the nanodisc-inserted histidine-tagged protein to a monoclonal anti-pentahistidine antibody was quantified using surface plasmon resonance (SPR) experiments. For the first time, a membrane-inserted transmembrane protein was employed as analyte while the antibody served as ligand immobilized on the sensor chip surface. SPR experiments were conducted in single-cycle mode. We demonstrate that the nanodisc-incorporated membrane protein showed nearly identical affinity toward the antibody as did the soluble decahistidine-tagged ubiquitin studied in a comparative experiment. Advantages of the new experimental setup and potential applications are discussed." @default.
- W2026240692 created "2016-06-24" @default.
- W2026240692 creator A5033755618 @default.
- W2026240692 creator A5066789895 @default.
- W2026240692 creator A5091520561 @default.
- W2026240692 date "2011-01-01" @default.
- W2026240692 modified "2023-09-27" @default.
- W2026240692 title "Nanodiscs allow the use of integral membrane proteins as analytes in surface plasmon resonance studies" @default.
- W2026240692 cites W1254448395 @default.
- W2026240692 cites W1521274794 @default.
- W2026240692 cites W1976291045 @default.
- W2026240692 cites W1977700439 @default.
- W2026240692 cites W1989590554 @default.
- W2026240692 cites W1989605828 @default.
- W2026240692 cites W1997199174 @default.
- W2026240692 cites W1997394451 @default.
- W2026240692 cites W2004620128 @default.
- W2026240692 cites W2005574282 @default.
- W2026240692 cites W2008504341 @default.
- W2026240692 cites W2010801138 @default.
- W2026240692 cites W2020340473 @default.
- W2026240692 cites W2022987595 @default.
- W2026240692 cites W2023814923 @default.
- W2026240692 cites W2026968105 @default.
- W2026240692 cites W2040785851 @default.
- W2026240692 cites W2040795711 @default.
- W2026240692 cites W2040941336 @default.
- W2026240692 cites W2041088894 @default.
- W2026240692 cites W2045109515 @default.
- W2026240692 cites W2051637056 @default.
- W2026240692 cites W2052772877 @default.
- W2026240692 cites W2062454332 @default.
- W2026240692 cites W2063868052 @default.
- W2026240692 cites W2076368535 @default.
- W2026240692 cites W2078034467 @default.
- W2026240692 cites W2079619903 @default.
- W2026240692 cites W2084426169 @default.
- W2026240692 cites W2085567137 @default.
- W2026240692 cites W2087299517 @default.
- W2026240692 cites W2090545415 @default.
- W2026240692 cites W2092371425 @default.
- W2026240692 cites W2099134954 @default.
- W2026240692 cites W2115150990 @default.
- W2026240692 cites W2120846499 @default.
- W2026240692 cites W2132820240 @default.
- W2026240692 cites W2152058099 @default.
- W2026240692 cites W2157774111 @default.
- W2026240692 cites W2160899469 @default.
- W2026240692 cites W2161572382 @default.
- W2026240692 cites W2170039100 @default.
- W2026240692 doi "https://doi.org/10.1016/j.ab.2010.08.028" @default.
- W2026240692 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20804721" @default.
- W2026240692 hasPublicationYear "2011" @default.
- W2026240692 type Work @default.
- W2026240692 sameAs 2026240692 @default.
- W2026240692 citedByCount "55" @default.
- W2026240692 countsByYear W20262406922012 @default.
- W2026240692 countsByYear W20262406922013 @default.
- W2026240692 countsByYear W20262406922014 @default.
- W2026240692 countsByYear W20262406922015 @default.
- W2026240692 countsByYear W20262406922016 @default.
- W2026240692 countsByYear W20262406922017 @default.
- W2026240692 countsByYear W20262406922018 @default.
- W2026240692 countsByYear W20262406922019 @default.
- W2026240692 countsByYear W20262406922020 @default.
- W2026240692 countsByYear W20262406922021 @default.
- W2026240692 countsByYear W20262406922022 @default.
- W2026240692 countsByYear W20262406922023 @default.
- W2026240692 crossrefType "journal-article" @default.
- W2026240692 hasAuthorship W2026240692A5033755618 @default.
- W2026240692 hasAuthorship W2026240692A5066789895 @default.
- W2026240692 hasAuthorship W2026240692A5091520561 @default.
- W2026240692 hasConcept C10390740 @default.
- W2026240692 hasConcept C106847996 @default.
- W2026240692 hasConcept C118892022 @default.
- W2026240692 hasConcept C12554922 @default.
- W2026240692 hasConcept C144647389 @default.
- W2026240692 hasConcept C155672457 @default.
- W2026240692 hasConcept C161200384 @default.
- W2026240692 hasConcept C170493617 @default.
- W2026240692 hasConcept C171250308 @default.
- W2026240692 hasConcept C185592680 @default.
- W2026240692 hasConcept C192562407 @default.
- W2026240692 hasConcept C24530287 @default.
- W2026240692 hasConcept C2780880673 @default.
- W2026240692 hasConcept C39944091 @default.
- W2026240692 hasConcept C41625074 @default.
- W2026240692 hasConcept C43617362 @default.
- W2026240692 hasConcept C55493867 @default.
- W2026240692 hasConcept C86803240 @default.
- W2026240692 hasConceptScore W2026240692C10390740 @default.
- W2026240692 hasConceptScore W2026240692C106847996 @default.
- W2026240692 hasConceptScore W2026240692C118892022 @default.
- W2026240692 hasConceptScore W2026240692C12554922 @default.
- W2026240692 hasConceptScore W2026240692C144647389 @default.
- W2026240692 hasConceptScore W2026240692C155672457 @default.
- W2026240692 hasConceptScore W2026240692C161200384 @default.
- W2026240692 hasConceptScore W2026240692C170493617 @default.