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• W2026310023 abstract "Neopterin is synthesized by human monocyte‐derived macrophages primarily upon stimulation with the cytokine interferon‐γ. We studied the influence of neopterin on the generation of reactive oxygen species (ROS) in human peripheral blood neutrophils. Radical formation was measured using a biochemiluminometer. Neutrophils were isolated from peripheral blood of healthy donors. The generation of ROS by neutrophils suspended in Earl's solution (pH=7.4) at 37°C was investigated by monitoring of chemiluminescence using luminol and lucigenin as light emitters. Neopterin induced chemiluminescence in suspensions of neutrophils in the presence of luminol, but not of lucigenin. Neopterin affected only adhesive cells. Addition of neopterin into the suspension of the cells involving D ‐mannitol, L ‐histidine and diazabicyclo[2.2.2]octane (DABCO) decreased luminol‐dependent chemiluminescence (LDCL) of the neutrophils. The action of superoxide dismutase (SOD) and 2‐phenyl‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl‐3‐oxide (PTIO) reduced neopterin‐induced LDCL of neutrophils. Data suggest that neutrophils respond on exposure to neopterin with additional generation of singlet oxygen, hydroxyl radical and nitric oxide by nicotinamide adenine dinucleotide phosphate (NADPH)‐independent pathways." @default.
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• W2026310023 date "2003-07-22" @default.
• W2026310023 modified "2023-09-25" @default.
• W2026310023 title "Influence of neopterin on the generation of reactive oxygen species in human neutrophils" @default.
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• W2026310023 doi "https://doi.org/10.1016/s0014-5793(03)00796-8" @default.
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