FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026334507> ?p ?o ?g. }

• W2026334507 endingPage "631" @default.
• W2026334507 startingPage "631" @default.
• W2026334507 abstract "In the yeast Saccharomyces cerevisiae, the enzyme gamma-glutamyl transpeptidase (gamma-GT; EC 2.3.2.2) is a glycoprotein that is bound to the vacuolar membrane. The kinetic parameters of GSH transport into isolated vacuoles were measured using intact vacuoles isolated from the wild-type yeast strain Sigma 1278b, under conditions of gamma-GT synthesis (nitrogen starvation) and repression (growth in the presence of ammonium ions). Vacuoles devoid of gamma-GT displayed a K(m) (app) of 18+/-2 mM and a V(max) (app) of 48.5+/-5 nmol of GSH/min per mg of protein. Vacuoles containing gamma-GT displayed practically the same K(m), but a higher V(max) (app) (150+/-12 nmol of GSH/min per mg of protein). Vacuoles prepared from a disruptant lacking gamma-GT showed no increase in V(max) (app) with nitrogen starvation. From a comparison of the transport data obtained for vacuoles isolated from various reference and mutant strains, it appears that the yeast cadmium factor 1 (YCF1) transport system accounts for approx. 70% of the GSH transport capacity of the vacuoles, the remaining 30% being due to a vacuolar (H(+)) ATPase-coupled system. The V(max) (app)-increasing effect of gamma-GT concerns only the YCF1 system. gamma-GT in the vacuolar membrane activates the Ycf1p transporter, either directly or indirectly. Moreover, GSH accumulating in the vacuolar space may exert a feedback effect on its own entry. Excretion of glutamate from radiolabelled GSH in isolated vacuoles containing gamma-GT was also measured. It is proposed that gamma-GT and a L-Cys-Gly dipeptidase catalyse the complete hydrolysis of GSH stored in the central vacuole of the yeast cell, prior to release of its constitutive amino acids L-glutamate, L-cysteine and glycine into the cytoplasm. Yeast appears to be a useful model for studying gamma-GT physiology and GSH metabolism." @default.
• W2026334507 created "2016-06-24" @default.
• W2026334507 creator A5062120331 @default.
• W2026334507 creator A5075235832 @default.
• W2026334507 creator A5081084822 @default.
• W2026334507 date "2001-11-01" @default.
• W2026334507 modified "2023-10-12" @default.
• W2026334507 title "γ-Glutamyl transpeptidase in the yeast Saccharomyces cerevisiae and its role in the vacuolar transport and metabolism of glutathione" @default.
• W2026334507 cites W1505266621 @default.
• W2026334507 cites W1510367374 @default.
• W2026334507 cites W1581416262 @default.
• W2026334507 cites W1584965218 @default.
• W2026334507 cites W1631725776 @default.
• W2026334507 cites W1682642099 @default.
• W2026334507 cites W1894211050 @default.
• W2026334507 cites W1900191660 @default.
• W2026334507 cites W1943243850 @default.
• W2026334507 cites W1967277910 @default.
• W2026334507 cites W1970749661 @default.
• W2026334507 cites W1975304761 @default.
• W2026334507 cites W1981957241 @default.
• W2026334507 cites W1993035984 @default.
• W2026334507 cites W2008608484 @default.
• W2026334507 cites W2034770144 @default.
• W2026334507 cites W2036941487 @default.
• W2026334507 cites W2042029123 @default.
• W2026334507 cites W2043643409 @default.
• W2026334507 cites W2059522016 @default.
• W2026334507 cites W2065158529 @default.
• W2026334507 cites W2094553079 @default.
• W2026334507 cites W2100057937 @default.
• W2026334507 cites W2100551137 @default.
• W2026334507 cites W2149887408 @default.
• W2026334507 cites W2150539008 @default.
• W2026334507 cites W2158714788 @default.
• W2026334507 cites W2167869241 @default.
• W2026334507 cites W2169357831 @default.
• W2026334507 cites W2264323239 @default.
• W2026334507 cites W278709392 @default.
• W2026334507 cites W4245957869 @default.
• W2026334507 doi "https://doi.org/10.1042/0264-6021:3590631" @default.
• W2026334507 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1222185" @default.
• W2026334507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11672438" @default.
• W2026334507 hasPublicationYear "2001" @default.
• W2026334507 type Work @default.
• W2026334507 sameAs 2026334507 @default.
• W2026334507 citedByCount "37" @default.
• W2026334507 countsByYear W20263345072012 @default.
• W2026334507 countsByYear W20263345072013 @default.
• W2026334507 countsByYear W20263345072014 @default.
• W2026334507 countsByYear W20263345072018 @default.
• W2026334507 countsByYear W20263345072019 @default.
• W2026334507 countsByYear W20263345072020 @default.
• W2026334507 countsByYear W20263345072021 @default.
• W2026334507 countsByYear W20263345072022 @default.
• W2026334507 crossrefType "journal-article" @default.
• W2026334507 hasAuthorship W2026334507A5062120331 @default.
• W2026334507 hasAuthorship W2026334507A5075235832 @default.
• W2026334507 hasAuthorship W2026334507A5081084822 @default.
• W2026334507 hasBestOaLocation W20263345072 @default.
• W2026334507 hasConcept C102568950 @default.
• W2026334507 hasConcept C181199279 @default.
• W2026334507 hasConcept C185592680 @default.
• W2026334507 hasConcept C190062978 @default.
• W2026334507 hasConcept C2777576037 @default.
• W2026334507 hasConcept C2779222958 @default.
• W2026334507 hasConcept C538909803 @default.
• W2026334507 hasConcept C55493867 @default.
• W2026334507 hasConcept C86803240 @default.
• W2026334507 hasConceptScore W2026334507C102568950 @default.
• W2026334507 hasConceptScore W2026334507C181199279 @default.
• W2026334507 hasConceptScore W2026334507C185592680 @default.
• W2026334507 hasConceptScore W2026334507C190062978 @default.
• W2026334507 hasConceptScore W2026334507C2777576037 @default.
• W2026334507 hasConceptScore W2026334507C2779222958 @default.
• W2026334507 hasConceptScore W2026334507C538909803 @default.
• W2026334507 hasConceptScore W2026334507C55493867 @default.
• W2026334507 hasConceptScore W2026334507C86803240 @default.
• W2026334507 hasIssue "3" @default.
• W2026334507 hasLocation W20263345071 @default.
• W2026334507 hasLocation W20263345072 @default.
• W2026334507 hasLocation W20263345073 @default.
• W2026334507 hasLocation W20263345074 @default.
• W2026334507 hasOpenAccess W2026334507 @default.
• W2026334507 hasPrimaryLocation W20263345071 @default.
• W2026334507 hasRelatedWork W1967277910 @default.
• W2026334507 hasRelatedWork W1975060682 @default.
• W2026334507 hasRelatedWork W2029793439 @default.
• W2026334507 hasRelatedWork W2062385823 @default.
• W2026334507 hasRelatedWork W2074767646 @default.
• W2026334507 hasRelatedWork W2078626599 @default.
• W2026334507 hasRelatedWork W2093521208 @default.
• W2026334507 hasRelatedWork W2397135598 @default.
• W2026334507 hasRelatedWork W2522656358 @default.
• W2026334507 hasRelatedWork W2572046523 @default.
• W2026334507 hasVolume "359" @default.
• W2026334507 isParatext "false" @default.
• W2026334507 isRetracted "false" @default.

• next