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- W2026337347 abstract "In serum-free primary culture of dissociated mouse epidermal cells, α-melanocyte stimulating hormone (α-MSH) and dibutyryl cyclic AMP (DBcAMP) induced the differentiation of melanocytes. Moreover, the proliferation of melanocytes was also induced in the dishes cultured with DBcAMP, but not with α-MSH. In order to clarify the role of keratinocytes in melanocyte proliferation and differentiation, pure cultures of keratinocytes were established in serum-free medium. Subconfluent primary keratinocytes were trypsinized and seeded into pure primary melanoblasts cultured with serum-free medium that did not contain α-MSH or DBcAMP. Melanoblasts were cultured with α-MSH or DBcAMP in the presence or absence of keratinocytes. α-MSH failed to induce melanocyte differentiation in the absence of keratinocytes. DBcAMP failed to induce melanocyte proliferation in the absence of keratinocytes, although it induced melanocyte differentiation even in the absence of keratinocytes. These results suggest that keratinocyte-derived factors are required not only for the induction of melanocyte differentiation by α-MSH but also for the induction of melanocyte proliferation by DBcAMP. © 1992 Wiley-Liss, Inc." @default.
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- W2026337347 date "1992-08-01" @default.
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- W2026337347 title "Melanocyte stimulating hormone induces the differentiation of mouse epidermal melanocytes in serum-free culture" @default.
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- W2026337347 doi "https://doi.org/10.1002/jcp.1041520215" @default.
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