FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026440935> ?p ?o ?g. }

• W2026440935 endingPage "305" @default.
• W2026440935 startingPage "293" @default.
• W2026440935 abstract "Grasspollen,especially of rye-grass (Lolium perenne), represents an important cause of type I allergy. Identification of IgE-binding (allergenic) epitopes of major grass pollen allergens is essential for understanding the molecular basis of interaction between allergens and human IgE antibodies and therefore facilitates the devising of safer and more effective diagnostic and immunotherapy reagents. The aim of this study was to identify the allergenic epitopes of Lol p 5, a major allergen of rye-grass pollen, immunodissect these epitopes further so that the amino acid residues critical for antibody binding can be determined and investigate the conservation and nature of these epitopes within the context of the natural grass pollen allergens. Peptides, 12 13 amino acid residues long and overlapping each other by 4 amino acid residues, based on the entire deduced amino acid sequence of the coding region of Lol p 5, were synthesised and assayed for IgE-binding. Two strong IgE-binding epitopes (Lol p 5 (49 60) and (265 276), referred to as peptides 7 and 34, respectively) were identified. These epitopes were further resolved by truncated peptides and amino acid replacement studies and the amino acid residues critical for IgE-binding determined (Lol p 5 (49 60) residue Lys57 and (265 276) residue Lys275 ). Sequences of these epitopes were conserved in related allergens and may form the conserved allergenic domains responsible for the cross-reactivity observed between pollen allergens of taxonomically related grasses. Furthermore, due to its strong IgE-reactivity, synthetic peptide Lol p 5 (265 276) was used to a.nity-purify specific IgE antibodies which recognised proteins of other clinically important grass pollens, further indicating presence of allergenic cross-reactivity at the level of allergenic epitope. Moreover, Lol p 5 (265 276) demonstrated a strong capacity to inhibit IgE-binding to natural rye-grass pollen proteins highlighting the antibody accessibility to these sequences within the context of the natural allergens. Strong IgE-binding epitopes of Lol p 5 have been identified down to single critical amino acid residues and are shown to occur as linear or continuous domains in the natural conformation of natural Lol p 5 and other group 5 grass pollen allergens. The fact that such an allergenic synthetic epitope has the capacity to strongly inhibit IgE-binding to natural allergens highlight its potential for use as a candidate in future therapeutics to treat pollen- associated allergies.© 1998 Elsevier Science Ltd. All rights reserved." @default.
• W2026440935 created "2016-06-24" @default.
• W2026440935 creator A5023421955 @default.
• W2026440935 creator A5028379393 @default.
• W2026440935 creator A5035660114 @default.
• W2026440935 creator A5044798622 @default.
• W2026440935 creator A5051080353 @default.
• W2026440935 creator A5052105106 @default.
• W2026440935 creator A5071610420 @default.
• W2026440935 creator A5072901780 @default.
• W2026440935 date "1998-04-01" @default.
• W2026440935 modified "2023-10-17" @default.
• W2026440935 title "Molecular basis of IgE-recognition of Lol p 5, a major allergen of rye-grass pollen" @default.
• W2026440935 cites W1491481694 @default.
• W2026440935 cites W1528139816 @default.
• W2026440935 cites W1535094031 @default.
• W2026440935 cites W170796376 @default.
• W2026440935 cites W1968906689 @default.
• W2026440935 cites W1998638985 @default.
• W2026440935 cites W2019898232 @default.
• W2026440935 cites W2032401333 @default.
• W2026440935 cites W2035923558 @default.
• W2026440935 cites W2046543747 @default.
• W2026440935 cites W2076363185 @default.
• W2026440935 cites W2079379693 @default.
• W2026440935 cites W2081739301 @default.
• W2026440935 cites W2082861534 @default.
• W2026440935 cites W2083102452 @default.
• W2026440935 cites W2095197150 @default.
• W2026440935 cites W2113520123 @default.
• W2026440935 cites W2113530505 @default.
• W2026440935 cites W2122581277 @default.
• W2026440935 cites W2195861155 @default.
• W2026440935 doi "https://doi.org/10.1016/s0161-5890(98)00050-9" @default.
• W2026440935 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9747889" @default.
• W2026440935 hasPublicationYear "1998" @default.
• W2026440935 type Work @default.
• W2026440935 sameAs 2026440935 @default.
• W2026440935 citedByCount "28" @default.
• W2026440935 countsByYear W20264409352012 @default.
• W2026440935 countsByYear W20264409352014 @default.
• W2026440935 countsByYear W20264409352017 @default.
• W2026440935 countsByYear W20264409352020 @default.
• W2026440935 crossrefType "journal-article" @default.
• W2026440935 hasAuthorship W2026440935A5023421955 @default.
• W2026440935 hasAuthorship W2026440935A5028379393 @default.
• W2026440935 hasAuthorship W2026440935A5035660114 @default.
• W2026440935 hasAuthorship W2026440935A5044798622 @default.
• W2026440935 hasAuthorship W2026440935A5051080353 @default.
• W2026440935 hasAuthorship W2026440935A5052105106 @default.
• W2026440935 hasAuthorship W2026440935A5071610420 @default.
• W2026440935 hasAuthorship W2026440935A5072901780 @default.
• W2026440935 hasConcept C104317684 @default.
• W2026440935 hasConcept C141105273 @default.
• W2026440935 hasConcept C159654299 @default.
• W2026440935 hasConcept C167625842 @default.
• W2026440935 hasConcept C185592680 @default.
• W2026440935 hasConcept C195616568 @default.
• W2026440935 hasConcept C203014093 @default.
• W2026440935 hasConcept C207480886 @default.
• W2026440935 hasConcept C2779142602 @default.
• W2026440935 hasConcept C2780510475 @default.
• W2026440935 hasConcept C2781338088 @default.
• W2026440935 hasConcept C3020064264 @default.
• W2026440935 hasConcept C515207424 @default.
• W2026440935 hasConcept C55493867 @default.
• W2026440935 hasConcept C86803240 @default.
• W2026440935 hasConceptScore W2026440935C104317684 @default.
• W2026440935 hasConceptScore W2026440935C141105273 @default.
• W2026440935 hasConceptScore W2026440935C159654299 @default.
• W2026440935 hasConceptScore W2026440935C167625842 @default.
• W2026440935 hasConceptScore W2026440935C185592680 @default.
• W2026440935 hasConceptScore W2026440935C195616568 @default.
• W2026440935 hasConceptScore W2026440935C203014093 @default.
• W2026440935 hasConceptScore W2026440935C207480886 @default.
• W2026440935 hasConceptScore W2026440935C2779142602 @default.
• W2026440935 hasConceptScore W2026440935C2780510475 @default.
• W2026440935 hasConceptScore W2026440935C2781338088 @default.
• W2026440935 hasConceptScore W2026440935C3020064264 @default.
• W2026440935 hasConceptScore W2026440935C515207424 @default.
• W2026440935 hasConceptScore W2026440935C55493867 @default.
• W2026440935 hasConceptScore W2026440935C86803240 @default.
• W2026440935 hasIssue "5" @default.
• W2026440935 hasLocation W20264409351 @default.
• W2026440935 hasLocation W20264409352 @default.
• W2026440935 hasOpenAccess W2026440935 @default.
• W2026440935 hasPrimaryLocation W20264409351 @default.
• W2026440935 hasRelatedWork W184119238 @default.
• W2026440935 hasRelatedWork W1908365524 @default.
• W2026440935 hasRelatedWork W2004802511 @default.
• W2026440935 hasRelatedWork W2039958282 @default.
• W2026440935 hasRelatedWork W2051192721 @default.
• W2026440935 hasRelatedWork W2067819394 @default.
• W2026440935 hasRelatedWork W2092258950 @default.
• W2026440935 hasRelatedWork W2135982139 @default.
• W2026440935 hasRelatedWork W2783630444 @default.
• W2026440935 hasRelatedWork W2944150887 @default.
• W2026440935 hasVolume "35" @default.

• next